Expression characterization of testicular DMRT1 in both Sertoli cells and spermatogenic cells of polyploid gibel carp

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	Expression characterization of testicular DMRT1 in both Sertoli cells and spermatogenic cells of polyploid gibel carp
	Li, Xi-Yin; Li, Zhi; Zhang, Xiao-Juan; Zhou, Li; Gui, Jian-Fang
刊名	GENE
	2014-09-10
卷号	548 期号:1 页码:119-125
关键词	Drrat1 Polyploid Gibel carp Sertoli cell Spermatogenesis Sex determination
ISSN号	0378-1119
英文摘要	Dmrt1 has been suggested to play significant roles in sex determination and differentiation, but various expression patterns and cell types have been observed in the testis of vertebrates. Polyploid gibel carp, because of the multiple modes of unisexual gynogenesis and sexual reproduction, has become a unique case to explore the evolution of sex determination and differentiation. However, the sex-determination related genes in gibel carp have remained unknown. In this study, we identified and characterized 4 cDNAs of Dmrt1 genes. Subsequently, a polyclonal antibody specific to CagDMRT1 was prepared to examine its expression and distribution patterns at protein level. Significantly, both relative real-time PCR and Western blot detection confirmed predominant expression of CagDmrt1 in the adult testis of gibel carp. Moreover, the intensive expression of CagDMRT1 around spermatogenic cysts was revealed during spermatogenesis. And, following immunofluorescence co-localization of CagDMRT1 and CagVASA, a prominent CagDMRT1 expression in Sertoli cells and a mild CagDMRT1 expression in spermatogenic cells including spermatogonia and primary spermatocytes were clearly characterized. The CagDMRT1 signal in Sertoli cells is extensively distributed in both nuclei and cytoplasm, while the CagDMRT1 in spermatogonia and primary spermatocytes is mainly expressed in nuclei, and there is only the remained CagDMRT1 signal in the cytoplasm of secondary spermatocytes. These findings suggest that DMRT1 should be related to testis differentiation and spermatogenesis in gibel carp. (C) 2014 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Genetics & Heredity
研究领域[WOS]	Genetics & Heredity
关键词[WOS]	CARASSIUS-AURATUS-GIBELIO ; SEX-DETERMINING GENES ; DM-DOMAIN GENE ; CORTICAL GRANULES ; XENOPUS-LAEVIS ; VERTEBRATE SEX ; MEDAKA FISH ; GERM-CELLS ; EVOLUTION ; ZEBRAFISH
收录类别	SCI
语种	英语
WOS记录号	WOS:000340329100017
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20338]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Li, Xi-Yin,Li, Zhi,Zhang, Xiao-Juan,et al. Expression characterization of testicular DMRT1 in both Sertoli cells and spermatogenic cells of polyploid gibel carp[J]. GENE,2014,548(1):119-125.
APA	Li, Xi-Yin,Li, Zhi,Zhang, Xiao-Juan,Zhou, Li,&Gui, Jian-Fang.(2014).Expression characterization of testicular DMRT1 in both Sertoli cells and spermatogenic cells of polyploid gibel carp.GENE,548(1),119-125.
MLA	Li, Xi-Yin,et al."Expression characterization of testicular DMRT1 in both Sertoli cells and spermatogenic cells of polyploid gibel carp".GENE 548.1(2014):119-125.