A second-generation genetic linkage map for bighead carp (Aristichthys nobilis) based on microsatellite markers

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	A second-generation genetic linkage map for bighead carp (Aristichthys nobilis) based on microsatellite markers
	Zhu, C.1,2; Tong, J.1; Yu, X.1; Guo, W.1,2; Wang, X.1,2; Liu, H.1,2; Feng, X.1,2; Sun, Y.1,2; Liu, L.1,2; Fu, B.2,3
刊名	ANIMAL GENETICS
	2014-10-01
卷号	45 期号:5 页码:699-708
关键词	Aristichthys nobilis genetic map interspecific hybrids pseudo-testcross simple sequence repeats
ISSN号	0268-9146
英文摘要	Bighead carp (Aristichthys nobilis) is an important aquaculture fish worldwide. Genetic linkage maps for the species were previously reported, but map resolution remained to be improved. In this study, a second-generation genetic linkage map was constructed for bighead carp through a pseudo-testcross strategy using interspecific hybrids between bighead carp and silver carp. Of the 754 microsatellites genotyped in two interspecific mapping families (with 77 progenies for each family), 659 markers were assigned to 24 linkage groups, which were equal to the chromosome numbers of the haploid genome. The consensus map spanned 1917.3cM covering 92.8% of the estimated bighead carp genome with an average marker interval of 2.9cM. The length of linkage groups ranged from 52.2 to 133.5cM with an average of 79.9cM. The number of markers per linkage group varied from 11 to 55 with an average of 27.5 per linkage group. Normality tests on interval distances of the map showed a non-normal marker distribution; however, significant correlation was found between the length of linkage group and the number of markers below the 0.01 significance level (two-tailed). The length of the female map was 1.12 times that of the male map, and the average recombination ratio of female to male was 1.10:1. Visual inspection showed that distorted markers gathered in some linkage groups and in certain regions of the male and female maps. This well-defined genetic linkage map will provide a basic framework for further genome mapping of quantitative traits, comparative mapping and marker-assisted breeding in bighead carp.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Agriculture, Dairy & Animal Science ; Genetics & Heredity
研究领域[WOS]	Agriculture ; Genetics & Heredity
关键词[WOS]	TROUT ONCORHYNCHUS-MYKISS ; CROSS-SPECIES APPLICATION ; HYPOPHTHALMICHTHYS-MOLITRIX ; RAINBOW-TROUT ; CTENOPHARYNGODON-IDELLA ; PARALICHTHYS-OLIVACEUS ; JAPANESE FLOUNDER ; ATLANTIC SALMON ; PACIFIC OYSTER ; AFLP MARKERS
收录类别	SCI
语种	英语
WOS记录号	WOS:000341682200010
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20333]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat CAS, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhu, C.,Tong, J.,Yu, X.,et al. A second-generation genetic linkage map for bighead carp (Aristichthys nobilis) based on microsatellite markers[J]. ANIMAL GENETICS,2014,45(5):699-708.
APA	Zhu, C..,Tong, J..,Yu, X..,Guo, W..,Wang, X..,...&Fu, B..(2014).A second-generation genetic linkage map for bighead carp (Aristichthys nobilis) based on microsatellite markers.ANIMAL GENETICS,45(5),699-708.
MLA	Zhu, C.,et al."A second-generation genetic linkage map for bighead carp (Aristichthys nobilis) based on microsatellite markers".ANIMAL GENETICS 45.5(2014):699-708.