Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway

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	Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway
	Wang, Bing; Zhang, Yi-Bing; Liu, Ting-Kai; Shi, Jun; Sun, Fan; Gui, Jian-Fang
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2014-11-01
卷号	47 期号:1 页码:140-149
关键词	Viperin Carassius auratus IFN inducible protein Promoter analysis RLR signaling pathway Antiviral function
ISSN号	0145-305X
英文摘要	Mammalian viperin is a typical interferon (IFN)-induced antiviral protein. Fish have viperin homologs; however, little is known about the expression regulation of fish viperins. In this study, we report the expression regulation and antiviral function of a fish viperin from crucian carp Carassius auratus during IFN response. Crucian carp viperin is induced at mRNA and protein levels by fish IFNs and IFN stimuli such as poly(I:C). Consistently, this gene promoter contains multiple transcription factor binding sites including IFN-stimulated response elements (ISRE) and IFN gamma activation sequences (GAS), and is activated by two types of fish IFNs and also by the intracellular and extracellular poly(I:C). Activation of crucian carp viperin promoter by the intracellular poly(I:C) is mediated by retinoic acid-inducing gene I (RIG-I)-like receptors (RLR)-triggered IFN signaling pathway, which is further verified by the findings that each signaling molecule of RLR pathway is able to induce the expression of crucian carp viperin at mRNA and protein levels. Finally, overexpression of crucian carp viperin in cultured fish cells confers significant protection against infection of grass carp reovirus (GCRV). These data suggest that similar to mammalian homologs, crucian carp viperin exerts a conserved function through RLR-triggered IFN signaling pathway. (C) 2014 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Immunology ; Zoology
研究领域[WOS]	Immunology ; Zoology
关键词[WOS]	INNATE IMMUNE-RESPONSE ; CARP CARASSIUS-AURATUS ; TROUT ONCORHYNCHUS-MYKISS ; DEPENDENT PROTEIN-KINASE ; HEPATITIS-C VIRUS ; GENE-EXPRESSION ; SUBCELLULAR-LOCALIZATION ; HUMAN CYTOMEGALOVIRUS ; MOLECULAR-CLONING ; DENDRITIC CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000342275400017
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20330]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wang, Bing,Zhang, Yi-Bing,Liu, Ting-Kai,et al. Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,47(1):140-149.
APA	Wang, Bing,Zhang, Yi-Bing,Liu, Ting-Kai,Shi, Jun,Sun, Fan,&Gui, Jian-Fang.(2014).Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,47(1),140-149.
MLA	Wang, Bing,et al."Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 47.1(2014):140-149.