Investigation of effect of 17 alpha-ethinylestradiol on vigilin expression using an isolated recombinant antibody

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Investigation of effect of 17 alpha-ethinylestradiol on vigilin expression using an isolated recombinant antibody
	Zhong, Liqiao 1; Yuan, Li 1; Rao, Yu 1; Li, Zhouquan 1,2; Gu, Qilin 1,2; Long, Yong 1; Zhang, Xiaohua 1; Cui, Zongbin 1; Xu, Ying 1; Dai, Heping 1
刊名	AQUATIC TOXICOLOGY
	2014-11-01
卷号	156 页码:1-9
关键词	Vigilin phage display Single-chain variable fragment (scFv) 17 alpha-ethinylestradiol (EE2) Zebrafish Crucian carp
ISSN号	0166-445X
英文摘要	Vigilin, a highly conserved protein from yeast to mammals, is a multifunctional protein in eukaryotic organisms. One biological function of vigilin is to stabilize the expression level of vitellogenin (VTG). This study aimed to develop vigilin as a new estrogen-inducible biomarker that correlates with the widely applied estrogen-inducible biomarker VTG and expand the ability to detect it in various species. Here, a recombinant monoclonal antibody with high specificity against the conserved C-terminal region of vigilin from zebrafish (Danio rerio) was successfully isolated from a phage display antibody library and found to recognize vigilin proteins from multiple vertebrate species. The effect of 17 alpha-ethinylestradiol (EE2) on vigilin expression in the liver of zebrafish and juvenile crucian carp (Carassius auratus) was investigated. Although vigilin mRNA was expressed in all tissues analyzed from male zebrafish, vigilin protein was detected exclusively in the testis of male zebrafish, as well as the liver of female zebrafish and juvenile crucian carp at a lower level without exposure to EE2. Significant induction of vigilin mRNA by exposure to EE2 was observed in the liver and testis of male zebrafish, even at a low dose of 6.25 ng/L (21.09 pmol/L). In Hela cells, the expression of vigilin coincided with high protein synthesis activity but not dose-dependently by EE2 exposure. Therefore, the recombinant antibody may be used as a detection tool to screen for mammalian cell lines or organs with estrogen-inducible expression of vigilin. (C) 2014 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Marine & Freshwater Biology ; Toxicology
研究领域[WOS]	Marine & Freshwater Biology ; Toxicology
关键词[WOS]	VITELLOGENIN MESSENGER-RNA ; KH-DOMAIN PROTEIN ; LIPOPROTEIN-BINDING-PROTEIN ; SACCHAROMYCES-CEREVISIAE ; ENDOPLASMIC-RETICULUM ; CHICKEN VIGILIN ; SCP160P ; YEAST ; SEQUENCE ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000345190000001
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20376]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhong, Liqiao,Yuan, Li,Rao, Yu,et al. Investigation of effect of 17 alpha-ethinylestradiol on vigilin expression using an isolated recombinant antibody[J]. AQUATIC TOXICOLOGY,2014,156:1-9.
APA	Zhong, Liqiao.,Yuan, Li.,Rao, Yu.,Li, Zhouquan.,Gu, Qilin.,...&Dai, Heping.(2014).Investigation of effect of 17 alpha-ethinylestradiol on vigilin expression using an isolated recombinant antibody.AQUATIC TOXICOLOGY,156,1-9.
MLA	Zhong, Liqiao,et al."Investigation of effect of 17 alpha-ethinylestradiol on vigilin expression using an isolated recombinant antibody".AQUATIC TOXICOLOGY 156(2014):1-9.