Expression pattern of somatolactin in the Chinese sturgeon, Acipenser sinensis Gray, 1835

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Expression pattern of somatolactin in the Chinese sturgeon, Acipenser sinensis Gray, 1835
	Leng, X. -Q.1; Li, C. -J.2; Cao, H.1
刊名	JOURNAL OF APPLIED ICHTHYOLOGY
	2014-12-01
卷号	30 期号:6 页码:1222-1228
关键词	MESSENGER-RNA EXPRESSION BREAM SPARUS-AURATA GROWTH-HORMONE PITUITARY PROTEIN CDNA CLONING MOLECULAR CHARACTERIZATION PHYLOGENETIC ANALYSIS FISH GONADOTROPINS RAINBOW-TROUT PLASMA-LEVELS
ISSN号	0175-8659
英文摘要	Somatolactin (SL) is a pituitary hormone specific to fish and is reported in a variety of teleost species. Although it appears to be involved in many different physiological processes, its biological functions are poorly understood in Acipenseriformes. In this paper the expression pattern of this protein is characterized in Chinese sturgeon (Acipenser sinensis). Structure analysis showed that AsSL had a 24aa signal peptide and a 208aa mature protein, one N-glycosylation site at positions 145-147 and seven Cys residues conserved with other SLs. The antiserum was raised against a 15 aa antigen, derived from the Chinese sturgeon somatolactin (AsSL) amino acid sequence. Western blot analysis showed the pituitary-specific expression of AsSL. In situ hybridization revealed that the mRNA of AsSL was expressed mainly in pars intermedia (PI), although also slightly in proximal pars distalis (PPD), cells expressing AsSL disperse throughout the pars intermedia. These findings provide useful information for future physiological research in Chinese sturgeon.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Marine & Freshwater Biology
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
关键词[WOS]	MESSENGER-RNA EXPRESSION ; BREAM SPARUS-AURATA ; GROWTH-HORMONE ; PITUITARY PROTEIN ; CDNA CLONING ; MOLECULAR CHARACTERIZATION ; PHYLOGENETIC ANALYSIS ; FISH GONADOTROPINS ; RAINBOW-TROUT ; PLASMA-LEVELS
收录类别	SCI ; ISTP
语种	英语
WOS记录号	WOS:000345588500018
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20375]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China 2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat & Utilizat, Agr Minist China, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Leng, X. -Q.,Li, C. -J.,Cao, H.. Expression pattern of somatolactin in the Chinese sturgeon, Acipenser sinensis Gray, 1835[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2014,30(6):1222-1228.
APA	Leng, X. -Q.,Li, C. -J.,&Cao, H..(2014).Expression pattern of somatolactin in the Chinese sturgeon, Acipenser sinensis Gray, 1835.JOURNAL OF APPLIED ICHTHYOLOGY,30(6),1222-1228.
MLA	Leng, X. -Q.,et al."Expression pattern of somatolactin in the Chinese sturgeon, Acipenser sinensis Gray, 1835".JOURNAL OF APPLIED ICHTHYOLOGY 30.6(2014):1222-1228.