Pause-melting misalignment: a novel model for the birth and motif indel of tandem repeats in the mitochondrial genome

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	Pause-melting misalignment: a novel model for the birth and motif indel of tandem repeats in the mitochondrial genome
	[Shi, Wei ; Kong, Xiao-Yu ; Wang, Zhong-Ming ; Yu, Shan-Shan] Chinese Acad Sci, Key Lab Marine Bioresource Sustainable Utilizat L, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China ; [Chen, Hai-Xia] Univ Gothenburg, Dept Biol & Environm Sci, SE-40530 Gothenburg, Sweden ; [De Stasio, Elizabeth A.] Lawrence Univ, Dept Biol, Appleton, WI 54911 USA
刊名	BMC GENOMICS
	2013
卷号	14 页码:-103
ISSN号	1471-2164
通讯作者	xykong@scsio.ac.cn
中文摘要	Background: Tandem repeats (TRs) in the mitochondrial (mt) genome control region have been documented in a wide variety of vertebrate species. The mechanism by which repeated tracts originate and undergo duplication and deletion, however, remains unclear. Results: We analyzed DNA sequences of mt genome TRs (mtTRs) in the ridged-eye flounder (Pleuronichthys cornutus), and characterized DNA sequences of mtTRs from other vertebrates using the data available in GenBank. Tandem repeats are concentrated in the control regions; however, we found approximately 16.6% of the TRs elsewhere in the mt genome. The flounder mtTRs possess three motif types with hypervariable characteristics at the 30 end of the control region (CR). Conclusion: Based on our analysis of this larger dataset of mtTR sequences, we propose a novel model of Pause Melting Misalignment (PMM) to describe the birth and motif indel of tandem repeats. PMM is activated during a pause event in mitochondrial replication in which a dynamic competition between the nascent (N) heavy strand and the displaced (D) heavy strand may lead to the melting of the N-strand from the template (T) light strand. When mispairing occurs during rebinding of the N-strand, one or several motifs can be inserted or deleted in both strands during the next round of mt-replication or repair. This model can explain the characteristics of TRs in available vertebrate mt genomes.
学科主题	Biotechnology & Applied Microbiology ; Genetics & Heredity
资助信息	This work was supported by the Natural Science Foundation of China (30870283, 31071890). We thank Prof. Ziniu Yu and Dr. Xiangyun Wu for critical review and advice.
原文出处	BIOMED CENTRAL LTD
语种	英语
WOS记录号	WOS:000316007600001
公开日期	2015-01-22
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/11069]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	[Shi, Wei,Kong, Xiao-Yu,Wang, Zhong-Ming,et al. Pause-melting misalignment: a novel model for the birth and motif indel of tandem repeats in the mitochondrial genome[J]. BMC GENOMICS,2013,14:-103.
APA	[Shi, Wei,Kong, Xiao-Yu,Wang, Zhong-Ming,Yu, Shan-Shan] Chinese Acad Sci, Key Lab Marine Bioresource Sustainable Utilizat L, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China,[Chen, Hai-Xia] Univ Gothenburg, Dept Biol & Environm Sci, SE-40530 Gothenburg, Sweden,&[De Stasio, Elizabeth A.] Lawrence Univ, Dept Biol, Appleton, WI 54911 USA.(2013).Pause-melting misalignment: a novel model for the birth and motif indel of tandem repeats in the mitochondrial genome.BMC GENOMICS,14,-103.
MLA	[Shi, Wei,et al."Pause-melting misalignment: a novel model for the birth and motif indel of tandem repeats in the mitochondrial genome".BMC GENOMICS 14(2013):-103.