An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication

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	An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication
	[Yan, Yang ; Guo, Chuanyu ; Huang, Xiaohong ; Wei, Jingguang ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Cui, Huachun] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA ; [Guo, Chuanyu] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Li, Jun] Sun Yat Sen Univ, Zhongshan Sch Med, Inst Human Virol, Guangzhou 510080, Guangdong, Peoples R China
刊名	JOURNAL OF GENERAL VIROLOGY
	2013
卷号	94 期号:12 页码:2759-2770
ISSN号	0022-1317
通讯作者	qinqw@scsio.ac.cn
中文摘要	Insulin-like growth factors (IGFs) play crucial roles in regulating cell differentiation, proliferation and apoptosis. In this study, a novel IGF homologue gene (IGF-like) encoded by Singapore grouper iridovirus (SGIV) ORF062R (termed SGIV-IGF), was cloned and characterized. The coding region of SGIV-IGF is 771 bp in length, with a variable number of tandem repeats (VNTR) locus at the 3'-end. We cloned one isoform of this novel gene, 582 bp in length, containing the predicted IGF domain and 3.6 copy numbers of the 27 bp repeat unit. SGIV-IGF was an early transcribed gene during viral infection, and SGIV-IGF was distributed predominantly in the cytoplasm with a diffused granular appearance. Intriguingly, overexpression of SGIV-IGF was able to promote the growth of grouper embryonic cells (GP cells) by promoting G1/S phase transition, which was at least partially dependent on its 3'-end VNTR locus. Furthermore, viral titre assay and real-time quantitative PCR (RT-qPCR) analysis proved that SGIV-IGF could promote SGIV replication in grouper cells. In addition, overexpression of SGIV-IGF mildly facilitated apoptosis in SGIV-infected non-host fathead minnow (FHM) cells. Together, our study demonstrated a novel functional gene of SGIV which may regulate viral replication and cellular processes through multiple mechanisms that appear to be cell type-dependent.
学科主题	Biotechnology & Applied Microbiology ; Virology
资助信息	This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213).
原文出处	SOC GENERAL MICROBIOLOGY
语种	英语
WOS记录号	WOS:000328808000021
公开日期	2015-01-22
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10955]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	[Yan, Yang,Guo, Chuanyu,Huang, Xiaohong,et al. An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication[J]. JOURNAL OF GENERAL VIROLOGY,2013,94(12):2759-2770.
APA	[Yan, Yang.,Guo, Chuanyu.,Huang, Xiaohong.,Wei, Jingguang.,Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.,...&[Li, Jun] Sun Yat Sen Univ, Zhongshan Sch Med, Inst Human Virol, Guangzhou 510080, Guangdong, Peoples R China.(2013).An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication.JOURNAL OF GENERAL VIROLOGY,94(12),2759-2770.
MLA	[Yan, Yang,et al."An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication".JOURNAL OF GENERAL VIROLOGY 94.12(2013):2759-2770.