A novel "Turn-On" fluorescent probe for F detection in aqueous solution and its application in live-cell imaging | |
Xu J(徐健)1,2,3![]() ![]() ![]() ![]() ![]() | |
刊名 | Analytica Chimica Acta
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2014 | |
卷号 | 849页码:36-42 |
关键词 | "Turn-On" fluorescent probe Fluoride ions sensing DFT calculation Live-cell imaging A549 and ATII cells |
ISSN号 | 0003-2670 |
通讯作者 | 邵士俊 ; 李应东 |
英文摘要 | A novel probe incorporating quaternized 4-pyridinium group into a BODIPY molecule was synthesized and studied for the selective detection of fluoride ions (F-) in aqueous solution. The design was based on a fluoride-specific desilylation reaction and the “Turn-On” fluorescent response of probe 1 to F- was ascribed to the inhibition of photoinduced electron transfer (PET) process. The probe displayed many desired properties such as high specificity, appreciable solubility, desirable response time and low toxicity to mammalian cells. There was a good linearity between the fluorescence intensity and the concentrations of F- in the range of 0.1–1 mM with a detection limit of 0.02 mM. The sensing mechanism was confirmed by the NMR, electrospray ionization mass spectrum, optical spectroscopy and the mechanism of “Turn-On” fluorescent response was also determinated by a density functional theory (DFT) calculation using Gaussian 03 program. Moreover, the probe was successfully applied for the fluorescence imaging of F- in human epithelial lung cancer (A549) cells and alveolar type II (ATII) cells under physiological conditions. |
学科主题 | 分析化学与药物化学 |
收录类别 | SCI |
资助信息 | the National Natural Science Foundation of China (20972170;21275150);the Funds for Distinguished Young Scientists of Gansu (1210RJDA013);the Natural Science Foundation of Gansu province (1107RJYA069) |
语种 | 英语 |
WOS记录号 | WOS:000343095900006 |
公开日期 | 2015-01-07 |
内容类型 | 期刊论文 |
源URL | [http://210.77.64.217/handle/362003/7100] ![]() |
专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
通讯作者 | Shao SJ(邵士俊) |
作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Gansu Univ Chinese Med, Inst Integrated Tradit & Western Med, Lanzhou 730000, Peoples R China |
推荐引用方式 GB/T 7714 | Xu J,Sun SB,Li Q,et al. A novel "Turn-On" fluorescent probe for F detection in aqueous solution and its application in live-cell imaging[J]. Analytica Chimica Acta,2014,849:36-42. |
APA | Xu J.,Sun SB.,Li Q.,Yue Y.,Li YD.,...&邵士俊.(2014).A novel "Turn-On" fluorescent probe for F detection in aqueous solution and its application in live-cell imaging.Analytica Chimica Acta,849,36-42. |
MLA | Xu J,et al."A novel "Turn-On" fluorescent probe for F detection in aqueous solution and its application in live-cell imaging".Analytica Chimica Acta 849(2014):36-42. |
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