A water-soluble BODIPY derivative as a highly selective "Turn-On" fluorescent sensor for H2O2 sensing in vivo

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	A water-soluble BODIPY derivative as a highly selective "Turn-On" fluorescent sensor for H2O2 sensing in vivo
	Xu J(徐健)1,2,3 ; Li Q(李欠)1,2 ; Yue Y(岳莹)1,2 ; Guo Y(郭勇)1,2 ; Shao SJ(邵士俊)1,2 ; Shao SJ(邵士俊)
刊名	Biosensors and Bioelectronics
	2014
卷号	56 页码:58-63
关键词	N-alkylpyridinium substituted BODIPY derivatives Water-soluble fluorescent probe Fluorescent sensor H2O2 In vitro and in vivo imaging
ISSN号	0956-5663
通讯作者	邵士俊
英文摘要	A type of BODIPY derivatives was designed and synthesized by the N-alkylation reaction of meso-(4-pyridinyl)-substituted BODIPY. The water-solubility of entire molecule was improved to a large extent as a result of the formation of cationic quaternary ammonium salt, while the strong fluorescence inherent to the BODIPY dye fragment is extinguished on alkylation of the pyridine N atom due to the photo- induced electron transfer (PET) process. The N-alkylated BODIPY derivative 4, as a novel water-soluble “Turn-On” fluorescent probe for the discrimination of H₂O₂, was constructed by incorporating 4-(Bromomethyl)benzeneboronic acid pinacol ester moiety, which showed highly selective fluorescent response to H₂O₂ against other interferences of ROS and RNS species under physiological conditions, and the reaction mechanism of boronate oxidation was confirmed by ¹H NMR, mass spectrum and optical spectroscopy analysis. As a biocompatible probe in biological systems, probe 4 was successfully applied for monitoring and imaging of H₂O₂ both in vitro and in vivo using HepG2/LO2 cells and angelfish.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the National Natural Science Foundation of China (20972170;21275150);the Funds for Distinguished Young Scientists of Gansu (1210RJDA013);the Natural Science Foundation of Gansu province (1107RJYA069)
语种	英语
WOS记录号	WOS:000333781000010
公开日期	2015-01-07
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/7099]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Shao SJ(邵士俊)
作者单位	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Xu J,Li Q,Yue Y,et al. A water-soluble BODIPY derivative as a highly selective "Turn-On" fluorescent sensor for H2O2 sensing in vivo[J]. Biosensors and Bioelectronics,2014,56:58-63.
APA	Xu J,Li Q,Yue Y,Guo Y,Shao SJ,&邵士俊.(2014).A water-soluble BODIPY derivative as a highly selective "Turn-On" fluorescent sensor for H2O2 sensing in vivo.Biosensors and Bioelectronics,56,58-63.
MLA	Xu J,et al."A water-soluble BODIPY derivative as a highly selective "Turn-On" fluorescent sensor for H2O2 sensing in vivo".Biosensors and Bioelectronics 56(2014):58-63.