Reversible immobilization of glucoamylase onto magnetic chitosan nanocarriers (SCI)

CORC > 寒区旱区环境与工程研究所 > 中科院寒区旱区环境与工程研究所（未分类） > 期刊论文

	Reversible immobilization of glucoamylase onto magnetic chitosan nanocarriers (SCI)
	Wang J. Z. ; Zhao G. H. ; Li Y. F. ; Liu X. ; Hou P. P.
刊名	Applied Microbiology and Biotechnology
	2013
卷号	97 期号:2 页码:681-692
ISSN号	ISBN/0175-7598
英文摘要	A simple preparation process for the monodispersed pH-sensitive core-shell magnetic microspheres was carried out consisting of chitosan self-assembled on magnetic iron oxide nanoparticles. Meanwhile, glucoamylase was immobilized as a model enzyme on this carrier of Fe3O4/CS microspheres by ionic adsorption. The morphology, inner structure, and high magnetic sensitivity of the resulting magnetic chitosan microspheres were studied, respectively, with a field emission scanning electron microscope (SEM), transmission electron microscope (TEM), FT-IR spectroscopy, thermogravimetric analysis (TGA), and a vibrating sample magnetometer (VSM). Subsequently, the properties of glucoamylase immobilized on the regenerated supports were also investigated by determining storage stability, pH stability, reusability, magnetic response, and regeneration of supports. The results from characterization and determination remarkably indicated that the immobilized glucoamylase obtained presents excellent storage stability, pH stability, reusability, magnetic response, and regeneration of supports. Therefore, this kind of magnetic Fe3O4/CS microspheres with perfect monodispersity should be an ideal support for enzyme immobilization.
收录类别	(SCI)
公开日期	2014-12-19
内容类型	期刊论文
源URL	[http://ir.casnw.net/handle/362004/23332]
专题	寒区旱区环境与工程研究所_中科院寒区旱区环境与工程研究所（未分类）_期刊论文
推荐引用方式 GB/T 7714	Wang J. Z.,Zhao G. H.,Li Y. F.,et al. Reversible immobilization of glucoamylase onto magnetic chitosan nanocarriers (SCI)[J]. Applied Microbiology and Biotechnology,2013,97(2):681-692.
APA	Wang J. Z.,Zhao G. H.,Li Y. F.,Liu X.,&Hou P. P..(2013).Reversible immobilization of glucoamylase onto magnetic chitosan nanocarriers (SCI).Applied Microbiology and Biotechnology,97(2),681-692.
MLA	Wang J. Z.,et al."Reversible immobilization of glucoamylase onto magnetic chitosan nanocarriers (SCI)".Applied Microbiology and Biotechnology 97.2(2013):681-692.