Regulating the mechanical properties of cells using a non-UV light-addressable hydrogel patterning process | |
Liu N(刘娜); Zhang CL(张常麟); Liu LQ(刘连庆)![]() ![]() ![]() | |
2014 | |
会议名称 | 2014 IEEE International Conference on Robotics and Automation (ICRA 2014) |
会议日期 | May 31 - June 7, 2014 |
会议地点 | HongKong, China |
页码 | 5866-5870 |
通讯作者 | 刘娜 |
中文摘要 | The determination of the mechanical properties of cells plays an important role in biological studies and has gained acceptance recently as a possible label-free biomarker for cell status determination or diseases detection. Investigations on how external cellular properties affect cell mechanics are helpful in understanding cell disease processes and cell morphogenesis, which are of large significance in medical science. Although most researchers have focused on individual cell mechanics, or the effect of substrate stiffness on cells, cell mechanical response due to interactions among cells is yet to be examined. A reason for this is that the study of cell mechanical response to cell shape requires one to use a cell patterning process. However, existing cell patterning methods are very complex and time-consuming. In this paper, we describe a practical and rapid technique that can easily pattern cells into desired shapes, which allows investigations of the effect of external environment on cell stiffness. In the new technique, Poly-(ethylene) glycol diacrylate (PEGDA) hydrogel film with thickness 70–100 nm is controllably patterned on a hydrogenated amorphous silicon (a-Si:H) substrate by polymerizing PEGDA molecules in-situ using programmable visual light patterns. The idea is to enable the confinement of cells cultured on the hydrogels into special areas. The elastic modulus of the patterned cells is measured using an atomic force microscope. Experimental results have demonstrated the versatility of the technique as a tool for cell pattering and exploration of cell mechanics under external mechanical stimuli. |
收录类别 | EI ; CPCI(ISTP) |
产权排序 | 1 |
会议录 | 2014 IEEE International Conference on Robotics and Automation (ICRA 2014)
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会议录出版者 | IEEE |
会议录出版地 | Piscataway, NJ, USA |
语种 | 英语 |
ISBN号 | 978-1-4799-3685-4 |
WOS记录号 | WOS:000377221105136 |
内容类型 | 会议论文 |
源URL | [http://ir.sia.cn/handle/173321/15393] ![]() |
专题 | 沈阳自动化研究所_机器人学研究室 |
推荐引用方式 GB/T 7714 | Liu N,Zhang CL,Liu LQ,et al. Regulating the mechanical properties of cells using a non-UV light-addressable hydrogel patterning process[C]. 见:2014 IEEE International Conference on Robotics and Automation (ICRA 2014). HongKong, China. May 31 - June 7, 2014. |
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