Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli

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	Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli
	Li, Xin 1,2; Han, Xu 2; Ko, Tzu-Ping 3; Chen, Chun-Chi 2; Zhu, Zhen 2; Hua, Erbing 1; Guo, Rey-Ting 2; Huang, Chun-Hsiang 2
刊名	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
	2013-03-01
卷号	69 期号:F69 页码:328-331
关键词	prenyltransferase octaprenyl pyrophosphate octaprenyl pyrophosphate synthase Escherichia coli
英文摘要	Octaprenyl pyrophosphate synthase (OPPs), which belongs to the E-type prenyltransferase family, catalyses the successive condensation of farnesyl pyrophosphate with five isopentenyl pyrophosphate molecules to form trans-C40-octaprenyl pyrophosphate (OPP). OPP is essential for the biosynthesis of bacterial ubiquinone or menaquinone side chains, which play an important role in the electron-transport system. Here, Escherichia coli OPPs was expressed, purified and crystallized. The crystals, which belonged to the orthorhombic space group P21212, with unit-cell parameters a = 117.0, b = 128.4, c = 46.4 angstrom, were obtained by the sitting-drop vapour-diffusion method and diffracted to 2.2 angstrom resolution. Initial phase determination by molecular replacement (MR) clearly indicated that the crystal contained one homodimer per asymmetric unit. Further model building and structural refinement are in progress.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Crystallography
关键词[WOS]	CHAIN-LENGTH DETERMINATION ; BIOSYNTHESIS ; ELONGATION ; MECHANISM ; SOFTWARE ; CRYSTALS ; MARITIMA ; ROLES ; SUITE
收录类别	SCI
语种	英语
WOS记录号	WOS:000316745000024
公开日期	2014-12-24
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/311007/552]
专题	天津工业生物技术研究所_结构生物学与蛋白酶学实验室郭瑞庭_期刊论文
作者单位	1.Tianjin Univ Sci & Technol, Tianjin Key Lab Ind Microbiol, Coll Biotechnol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Ind Enzymes Natl Engn Lab, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China 3.Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan
推荐引用方式 GB/T 7714	Li, Xin,Han, Xu,Ko, Tzu-Ping,et al. Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2013,69(F69):328-331.
APA	Li, Xin.,Han, Xu.,Ko, Tzu-Ping.,Chen, Chun-Chi.,Zhu, Zhen.,...&Huang, Chun-Hsiang.(2013).Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,69(F69),328-331.
MLA	Li, Xin,et al."Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 69.F69(2013):328-331.