| Structural Insights into Enzymatic Degradation of Oxidized Polyvinyl Alcohol | |
| Yang, Yu1; Ko, Tzu-Ping3; Liu, Long1; Li, Jianghua1; Huang, Chun-Hsiang2; Chan, Hsiu-Chien2; Ren, Feifei2; Jia, Dongxu1; Wang, Andrew H. -J.3; Guo, Rey-Ting2 | |
| 刊名 | CHEMBIOCHEM
 |
| 2014-09-05 | |
| 卷号 | 15期号:13页码:1882-1886 |
| 关键词 | catalytic triad beta-diketone hydrolases double oxyanion holes environmental chemistry hydrolases microbial assimilation |
| 英文摘要 | The ever-increasing production and use of polyvinyl alcohol (PVA) threaten our environment. Yet PVA can be assimilated by microbes in two steps: oxidation and cleavage. Here we report novel a/b-hydrolase structures of oxidized PVA hydrolase (OPH) from two known PVA-degrading organisms, Sphingopyxis sp. 113P3 and Pseudomonas sp. VM15C, including complexes with substrate analogues, acetylacetone and caprylate. The active site is covered by a lid-like beta-ribbon. Unlike other esterase and amidase, OPH is unique in cleaving the C = C bond of beta-diketone, although it has a catalytic triad similar to that of most alpha/beta-hydrolases. Analysis of the crystal structures suggests a double-oxyanion-hole mechanism, previously only found in thiolase cleaving beta-ketoacyl-CoA. Three mutations in the lid region showed enhanced activity, with potential in industrial applications. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Medicinal |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 关键词[WOS] | STRUCTURE REFINEMENT ; CRYSTAL-STRUCTURES ; CRYSTALLOGRAPHY ; HYDROLASE ; SOFTWARE ; 113P3 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341586100005 |
| 公开日期 | 2014-12-23 |
| 内容类型 | 期刊论文 |
| 源URL | [http://124.16.173.210/handle/311007/534]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Jiangnan Univ, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Peoples R China 2.Chinese Acad Sci, Tianjin Airport Econ Area, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 3.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan |
| 推荐引用方式 GB/T 7714 | Yang, Yu,Ko, Tzu-Ping,Liu, Long,et al. Structural Insights into Enzymatic Degradation of Oxidized Polyvinyl Alcohol[J]. CHEMBIOCHEM,2014,15(13):1882-1886. |
| APA | Yang, Yu.,Ko, Tzu-Ping.,Liu, Long.,Li, Jianghua.,Huang, Chun-Hsiang.,...&Du, Guocheng.(2014).Structural Insights into Enzymatic Degradation of Oxidized Polyvinyl Alcohol.CHEMBIOCHEM,15(13),1882-1886. |
| MLA | Yang, Yu,et al."Structural Insights into Enzymatic Degradation of Oxidized Polyvinyl Alcohol".CHEMBIOCHEM 15.13(2014):1882-1886. |
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