A novel and rapid HPGPC-based strategy for quality control of saccharide-dominant herbal materials: Dendrobium officinale, a case study

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	A novel and rapid HPGPC-based strategy for quality control of saccharide-dominant herbal materials: Dendrobium officinale, a case study
	Xu, Jun1 ; Li, Song-Lin 2,3; Yue, Rui-Qi 1; Ko, Chun-Hay 4; Hu, Jiang-Miao5 ; Liu, Jing 1; Ho, Hing-Man 1; Yi, Tao 1; Zhao, Zhong-Zhen 1; Zhou, Jun5
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2014-10-01
卷号	406 期号:25 页码:6409-6417
关键词	HPGPC fingerprints Saccharide-dominant herbal materials Holistic polysaccharide marker Quality evaluation Dendrobium officinale
ISSN号	1618-2642
通讯作者	Chen,HB (reprint author),Hong Kong Baptist Univ,Sch Chinese Med,7 Baptist Univ Rd,Kowloon Tong,Hong Kong,Peoples R China. ; hbchen@hkbu.edu.hk ; simonhan@hkbu.edu.hk
产权排序	第五
英文摘要	Qualitative and quantitative characterization of natural saccharides, especially polysaccharides, in herb materials remains a challenge due to their complicated structures and high macromolecular masses. Currently available methods involve time-consuming and complicated operations, and present poor specificity. Here, a novel and rapid high-performance gel permeation chromatography (HPGPC)-based approach is described for quality assessment of saccharide-dominant herbal materials by simultaneous qualitative and quantitative analysis of saccharide components. Dendrobium officinale, one of the rarest tonic herbs worldwide, was employed as the model herb in this study. First, a HPGPC fingerprint based on the molecular weight distribution of its carbohydrate components was established for qualitative identification of D. officinale. Then, HPGPC-guided dominant holistic polysaccharide marker was separated using ultra-filtration followed by HPGPC determination for quantitative evaluation of D. officinale. The experimental results suggest that this method is more efficient, stable, and convenient compared with the currently available methods for authentication and quality evaluation of D. officinale, and we expect the method will have similar advantages when used for quality control of other saccharide-dominant herbal materials and products.
学科主题	Biochemistry & Molecular Biology ; Chemistry
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	GAS-CHROMATOGRAPHY ; POLYSACCHARIDES ; SUGARS ; RADIX
收录类别	SCI
资助信息	Hong Kong Baptist University [FRG2/11-12/048, FRG1/12-13/018, FRG2/12-13/006, FRG2/13-14/026, MPCF-001-2014/2015]; Hong Kong Baptist University (RC-start up grant)
语种	英语
WOS记录号	WOS:000342224300031
公开日期	2014-11-25
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/18380]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon Tong, Hong Kong, Peoples R China 2.China Acad ChineseMed Sci, Dept Pharmaceut Anal & Metab, Jiangsu Branch, Nanjing, Jiangsu, Peoples R China 3.Jiangsu Prov Acad Tradit Chinese Med, Nanjing, Jiangsu, Peoples R China 4.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Inst Chinese Med, Shatin, Hong Kong, Peoples R China 5.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Xu, Jun,Li, Song-Lin,Yue, Rui-Qi,et al. A novel and rapid HPGPC-based strategy for quality control of saccharide-dominant herbal materials: Dendrobium officinale, a case study[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2014,406(25):6409-6417.
APA	Xu, Jun.,Li, Song-Lin.,Yue, Rui-Qi.,Ko, Chun-Hay.,Hu, Jiang-Miao.,...&Han, Quan-Bin.(2014).A novel and rapid HPGPC-based strategy for quality control of saccharide-dominant herbal materials: Dendrobium officinale, a case study.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,406(25),6409-6417.
MLA	Xu, Jun,et al."A novel and rapid HPGPC-based strategy for quality control of saccharide-dominant herbal materials: Dendrobium officinale, a case study".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 406.25(2014):6409-6417.