Effects of Simulated Microgravity on Embryonic Stem Cells

	Effects of Simulated Microgravity on Embryonic Stem Cells
	Wang, Yulan; An, Lili; Jiang, Yuanda; Hang, Haiying
刊名	PLOS ONE
	2011
卷号	6 期号:12 页码:e29214
ISSN号	1932-6203
通讯作者	Wang, YL (reprint author), Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China.
中文摘要	There have been many studies on the biological effects of simulated microgravity (SMG) on differentiated cells or adult stem cells. However, there has been no systematic study on the effects of SMG on embryonic stem (ES) cells. In this study, we investigated various effects (including cell proliferation, cell cycle distribution, cell differentiation, cell adhesion, apoptosis, genomic integrity and DNA damage repair) of SMG on mouse embryonic stem (mES) cells. Mouse ES cells cultured under SMG condition had a significantly reduced total cell number compared with cells cultured under 1 g gravity (1G) condition. However, there was no significant difference in cell cycle distribution between SMG and 1G culture conditions, indicating that cell proliferation was not impaired significantly by SMG and was not a major factor contributing to the total cell number reduction. In contrast, a lower adhesion rate cultured under SMG condition contributed to the lower cell number in SMG. Our results also revealed that SMG alone could not induce DNA damage in mES cells while it could affect the repair of radiation-induced DNA lesions of mES cells. Taken together, mES cells were sensitive to SMG and the major alterations in cellular events were cell number expansion, adhesion rate decrease, increased apoptosis and delayed DNA repair progression, which are distinct from the responses of other types of cells to SMG.
英文摘要	There have been many studies on the biological effects of simulated microgravity (SMG) on differentiated cells or adult stem cells. However, there has been no systematic study on the effects of SMG on embryonic stem (ES) cells. In this study, we investigated various effects (including cell proliferation, cell cycle distribution, cell differentiation, cell adhesion, apoptosis, genomic integrity and DNA damage repair) of SMG on mouse embryonic stem (mES) cells. Mouse ES cells cultured under SMG condition had a significantly reduced total cell number compared with cells cultured under 1 g gravity (1G) condition. However, there was no significant difference in cell cycle distribution between SMG and 1G culture conditions, indicating that cell proliferation was not impaired significantly by SMG and was not a major factor contributing to the total cell number reduction. In contrast, a lower adhesion rate cultured under SMG condition contributed to the lower cell number in SMG. Our results also revealed that SMG alone could not induce DNA damage in mES cells while it could affect the repair of radiation-induced DNA lesions of mES cells. Taken together, mES cells were sensitive to SMG and the major alterations in cellular events were cell number expansion, adhesion rate decrease, increased apoptosis and delayed DNA repair progression, which are distinct from the responses of other types of cells to SMG.
学科主题	空间技术
收录类别	SCI
资助信息	Chinese Academy of Sciences [KSCX2-YW-R63]
语种	英语
公开日期	2014-12-15
内容类型	期刊论文
源URL	[http://ir.nssc.ac.cn/handle/122/3007]
专题	国家空间科学中心_空间技术部
推荐引用方式 GB/T 7714	Wang, Yulan,An, Lili,Jiang, Yuanda,et al. Effects of Simulated Microgravity on Embryonic Stem Cells[J]. PLOS ONE,2011,6(12):e29214.
APA	Wang, Yulan,An, Lili,Jiang, Yuanda,&Hang, Haiying.(2011).Effects of Simulated Microgravity on Embryonic Stem Cells.PLOS ONE,6(12),e29214.
MLA	Wang, Yulan,et al."Effects of Simulated Microgravity on Embryonic Stem Cells".PLOS ONE 6.12(2011):e29214.