Analysis and Evaluation of Fatty Acid in Rattan Tea by Highly Selective and Sensitive HPLC-FLD-MS Method Coupled with Pre-Column Fluorescent Labeling

	Analysis and Evaluation of Fatty Acid in Rattan Tea by Highly Selective and Sensitive HPLC-FLD-MS Method Coupled with Pre-Column Fluorescent Labeling
	Fan, Baolei 1,2,3; Chen, Guang 1,3; Chen, Xiangming 1,3; Suo, Yourui 1
刊名	asian journal of chemistry
	2014
卷号	26 期号:1 页码:103-109
关键词	Fatty acids Rattan Tea RE-HPLC-APCI/MS Fluorescence detection Pre-column derivatization
ISSN号	0970-7077
中文摘要	rattan tea regarded as one of the most promising dietary supplements for the prevention and treatment of many chronic diseases has gained extensive attention. free fatty acids play an important role in human body as essential nutrients. contents of 31 free fatty acids in rattan tea were firstly investigated using toluene-4-sulfonic acid 2-(naphthalene-1-yl-phenyl-amino)-ethyl ester as pre-column fluorescence derivatization reagent by re-hplc coupled with an atmospheric chemical ionization-mass spectrometry source (apci/ ms). the results indicated that lower values of lod (0.52-1.53 ng ml(-1) ) provided the guarantee for trace analysis and evaluation of free fatty acids. the experimental recoveries were in the range of 88.9-103.6 %. results of the present study indicated the highest free fatty acids content of rattan tea was in april. thirteen free fatty acids were found, including c18:2 omega 6, c18:3 omega 3, omega 6 and c20:4 omega 6 essential to life. the developed method exhibited powerful potential for the systematic detection of bioactive components of medicinal plants.
英文摘要	rattan tea regarded as one of the most promising dietary supplements for the prevention and treatment of many chronic diseases has gained extensive attention. free fatty acids play an important role in human body as essential nutrients. contents of 31 free fatty acids in rattan tea were firstly investigated using toluene-4-sulfonic acid 2-(naphthalene-1-yl-phenyl-amino)-ethyl ester as pre-column fluorescence derivatization reagent by re-hplc coupled with an atmospheric chemical ionization-mass spectrometry source (apci/ ms). the results indicated that lower values of lod (0.52-1.53 ng ml(-1) ) provided the guarantee for trace analysis and evaluation of free fatty acids. the experimental recoveries were in the range of 88.9-103.6 %. results of the present study indicated the highest free fatty acids content of rattan tea was in april. thirteen free fatty acids were found, including c18:2 omega 6, c18:3 omega 3, omega 6 and c20:4 omega 6 essential to life. the developed method exhibited powerful potential for the systematic detection of bioactive components of medicinal plants.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	gas-chromatography ; phenolic-compounds ; mass-spectrometry ; food-chain ; quantification ; identification ; reagent ; leaves ; lipids ; uv
收录类别	SCI
语种	英语
WOS记录号	WOS:000330294400024
公开日期	2014-12-19
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/4286]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai Provinc, Peoples R China 2.Hubei Univ Sci & Technol, Xianning, Hubei Provice, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Fan, Baolei,Chen, Guang,Chen, Xiangming,et al. Analysis and Evaluation of Fatty Acid in Rattan Tea by Highly Selective and Sensitive HPLC-FLD-MS Method Coupled with Pre-Column Fluorescent Labeling[J]. asian journal of chemistry,2014,26(1):103-109.
APA	Fan, Baolei,Chen, Guang,Chen, Xiangming,&Suo, Yourui.(2014).Analysis and Evaluation of Fatty Acid in Rattan Tea by Highly Selective and Sensitive HPLC-FLD-MS Method Coupled with Pre-Column Fluorescent Labeling.asian journal of chemistry,26(1),103-109.
MLA	Fan, Baolei,et al."Analysis and Evaluation of Fatty Acid in Rattan Tea by Highly Selective and Sensitive HPLC-FLD-MS Method Coupled with Pre-Column Fluorescent Labeling".asian journal of chemistry 26.1(2014):103-109.