Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography | |
He, Yanfeng1,2; Lv, Huanhuan1,2; Wang, Xiaoyan1; Suo, Yourui1; Wang, Honglun1 | |
刊名 | separation science and technology
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2014-01-31 | |
卷号 | 49期号:4页码:580-587 |
关键词 | flavonoids high-speed counter-current chromatography Trigonella foenum-graecum L preparative isolation polyhydroxystilbenes |
ISSN号 | 0149-6395 |
中文摘要 | it is difficult to simultaneously isolate and purify flavonoids and polyhydroxystilbenes due to their similar polarities. in this study, we isolated and purified these compounds from trigonella foenum-graecum l. seed extracts using high-speed counter-current chromatography (hsccc) in two steps with two different kinds of solvent systems. in the first step, n-hexane:ethyl acetate:methanol:water (1:5:1:5, v/v) was used as the two-phase solvent system, desoxy-rhaponticin and rhapontigenin were purified, and orientin, vitexin, isovitexin, and rhaponticin were eluted together. in the second step, ethyl acetate:water (1:1, v/v) was used as the two-phase solvent system, and orientin, vitexin, rhaponticin, and isovitexin were isolated and purified. purities of the isolated compounds were determined using hplc, while uv spectra, h- 1 -nmr, and c- 13 -nmr aided in structure identification. |
英文摘要 | it is difficult to simultaneously isolate and purify flavonoids and polyhydroxystilbenes due to their similar polarities. in this study, we isolated and purified these compounds from trigonella foenum-graecum l. seed extracts using high-speed counter-current chromatography (hsccc) in two steps with two different kinds of solvent systems. in the first step, n-hexane:ethyl acetate:methanol:water (1:5:1:5, v/v) was used as the two-phase solvent system, desoxy-rhaponticin and rhapontigenin were purified, and orientin, vitexin, isovitexin, and rhaponticin were eluted together. in the second step, ethyl acetate:water (1:1, v/v) was used as the two-phase solvent system, and orientin, vitexin, rhaponticin, and isovitexin were isolated and purified. purities of the isolated compounds were determined using hplc, while uv spectra, h- 1 -nmr, and c- 13 -nmr aided in structure identification. |
WOS标题词 | science & technology ; physical sciences ; technology |
类目[WOS] | chemistry, multidisciplinary ; engineering, chemical |
研究领域[WOS] | chemistry ; engineering |
关键词[WOS] | preparative separation ; antioxidant activities ; solvent systems ; fenugreek seeds ; rheum-undulatum ; rhapontigenin ; extract ; damage ; mice |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000331526700012 |
公开日期 | 2014-12-19 |
内容类型 | 期刊论文 |
源URL | [http://ir.nwipb.ac.cn/handle/363003/4261] ![]() |
专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | He, Yanfeng,Lv, Huanhuan,Wang, Xiaoyan,et al. Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography[J]. separation science and technology,2014,49(4):580-587. |
APA | He, Yanfeng,Lv, Huanhuan,Wang, Xiaoyan,Suo, Yourui,&Wang, Honglun.(2014).Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography.separation science and technology,49(4),580-587. |
MLA | He, Yanfeng,et al."Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography".separation science and technology 49.4(2014):580-587. |
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