Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells

	Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells
	Wang, Yi 1; Nie, Fangyuan 1; Ouyang, Jian 2; Wang, Xiaoyan 2; Ma, Xiaofeng 1
刊名	tumor biology
	2014-10-01
卷号	35 期号:10 页码:9563-9569
关键词	Fatty acid synthase Inhibitor Sea buckthorn Procyanidins MDA-MB-231 cells Cell apoptosis
ISSN号	1010-4283
中文摘要	fatty acid synthase (fas) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. sea buckthorn has long been used to treat a variety of maladies. here, we investigated the inhibitory effect of sea buckthorn procyanidins (sbps) isolated from the seeds of sea buckthorn on fas and fas overexpressed human breast cancer mda-mb-231 cells. the fas activity and fas inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (nadph) absorption. we found that sbp potently inhibited the activity of fas with a half-inhibitory concentration (ic50) value of 0.087 mu g/ml. 3-4,5-dimethylthiazol- 2-yl-2,3-diphenyl tetrazolium bromide (mtt) assay was used to test the cell viability. sbp reduced mda-mb-231 cell viability with an ic50 value of 37.5 mu g/ml. hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that sbp induced mda-mb-231 cell apoptosis. sbp inhibited intracellular fas activity with a dose-dependent manner. in addition, sodium palmitate could rescue the cell apoptosis induced by sbp. these results showed that sbp was a promising fas inhibitor which could induce the apoptosis of mda-mb-231 cells via inhibiting fas. these findings suggested that sbp might be useful for preventing or treating breast cancer.
英文摘要	fatty acid synthase (fas) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. sea buckthorn has long been used to treat a variety of maladies. here, we investigated the inhibitory effect of sea buckthorn procyanidins (sbps) isolated from the seeds of sea buckthorn on fas and fas overexpressed human breast cancer mda-mb-231 cells. the fas activity and fas inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (nadph) absorption. we found that sbp potently inhibited the activity of fas with a half-inhibitory concentration (ic50) value of 0.087 mu g/ml. 3-4,5-dimethylthiazol- 2-yl-2,3-diphenyl tetrazolium bromide (mtt) assay was used to test the cell viability. sbp reduced mda-mb-231 cell viability with an ic50 value of 37.5 mu g/ml. hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that sbp induced mda-mb-231 cell apoptosis. sbp inhibited intracellular fas activity with a dose-dependent manner. in addition, sodium palmitate could rescue the cell apoptosis induced by sbp. these results showed that sbp was a promising fas inhibitor which could induce the apoptosis of mda-mb-231 cells via inhibiting fas. these findings suggested that sbp might be useful for preventing or treating breast cancer.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	oncology
研究领域[WOS]	oncology
关键词[WOS]	seabuckthorn hippophae-rhamnoides ; induced oxidative stress ; nf-kappa-b ; cancer-cells ; epigallocatechin gallate ; antitumor-activity ; molecular targets ; natural inhibitor ; prostate-cancer ; palmitic acid
收录类别	SCI
语种	英语
WOS记录号	WOS:000344532700014
公开日期	2014-12-19
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/4181]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai Provinc, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yi,Nie, Fangyuan,Ouyang, Jian,et al. Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells[J]. tumor biology,2014,35(10):9563-9569.
APA	Wang, Yi,Nie, Fangyuan,Ouyang, Jian,Wang, Xiaoyan,&Ma, Xiaofeng.(2014).Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells.tumor biology,35(10),9563-9569.
MLA	Wang, Yi,et al."Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells".tumor biology 35.10(2014):9563-9569.