Stratifi ed polymer brushes are fabricated using microcontact printing (μCP) of initiator integrated polydopamine (PDOPBr) on polymer brush surfaces and the following surface initiated atom transfer radical polymerization (SI-ATRP). It is found that the surface energy, chemically active groups, and the antifouling ability of the polymer brushes affect transfer effi ciency and adhesive stability of the polydopamine fi lm. The stickiness of the PDOPBr pattern on polymer brush surfaces is stable enough to perform continuous μCP and SI-ATRP to prepare stratifi ed polymer brushes with a 3D topography, which have broad applications in cell and protein patterning, biosensors, and hybrid surfaces.