Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection

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	Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection
	Wei, SN ; Huang, YH ; Huang, XH ; Cai, J ; Yan, Y ; Guo, CY ; Qin, QW
刊名	FISH & SHELLFISH IMMUNOLOGY
	2014
卷号	36 期号:1 页码:194-205
关键词	Epinephelus coioides Cathepsin B Singapore grouper iridovirus (SGIV) Virus replication Apoptosis
ISSN号	1050-4648
通讯作者	qinqw@scsio.ac.cn
中文摘要	The lysosomal cysteine protease cathepsin B of papain family is a key regulator and signaling molecule that involves in various biological processes, such as the regulation of apoptosis and activation of virus. In the present study, cathepsin B gene (Ec-CB) was cloned and characterized from orange-spotted grouper, Epinephelus coioides. The full-length Ec-CB cDNA was composed of 1918 bp and encoded a polypeptide of 330 amino acids with higher identities to cathepsin B of teleosts and mammalians. Ec-CB possessed typical cathepsin B structural features including an N-terminal signal peptide, the propeptide region and the cysteine protease domain which were conserved in other cathepsin B sequences. Phylogenetic analysis revealed that Ec-CB was most closely related to Lutjanus argentimaculatus. RT-PCR analysis showed that Ec-CB transcript was expressed in all the examined tissues which abundant in spleen, kidney and gill. After challenged with Singapore grouper iridovirus (SGIV) stimulation, the mRNA expression of cathepsin B in E. coioides was up-regulated at 24 h post-infection. Subcellular localization analysis revealed that Ec-CB was distributed predominantly in the cytoplasm. When the fish cells (GS or FHM) were treated with the cathepsin B specific inhibitor CA-074Me, the occurrence of CPE induced by SGIV was delayed, and the viral gene transcription was significantly inhibited. Additionally, SGIV-induced typical apoptosis was also inhibited by CA-074Me in FHM cells. Taken together, our results demonstrated that the Ec-CB might play a functional role in SGIV infection. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
资助信息	National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070]; knowledge innovation program of the Chinese Academy of Sciences [KZCX2-EW-Q213, KZCX2-YW-BR-08]
原文出处	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000331671600024
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10493]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Wei, SN,Huang, YH,Huang, XH,et al. Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection[J]. FISH & SHELLFISH IMMUNOLOGY,2014,36(1):194-205.
APA	Wei, SN.,Huang, YH.,Huang, XH.,Cai, J.,Yan, Y.,...&Qin, QW.(2014).Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection.FISH & SHELLFISH IMMUNOLOGY,36(1),194-205.
MLA	Wei, SN,et al."Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection".FISH & SHELLFISH IMMUNOLOGY 36.1(2014):194-205.