IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis
	Fu, JP ; Chen, SN ; Zou, PF ; Huang, B ; Guo, Z ; Zeng, LB ; Qin, QW ; Nie, P
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2014
卷号	43 期号:1 页码:87-95
关键词	IFN-gamma IFN-gamma stimulated gene Iridovirus Antiviral activity Turtle
ISSN号	0145-305X
通讯作者	pinnie@ihb.ac.cn
中文摘要	The IFN-gamma gene was identified in a turtle, the Chinese soft-shelled turtle, Pelodiscus sinensis, with its genome consisting of 4 exons and 3 introns. The deduced amino acid sequence of this gene contains a signal peptide, an IFN-gamma family signature motif (130)IQRKAVNELFPT, an NLS motif (KRKR)-K-155 and three potential N-glycosylation sites. As revealed by real-time quantitative PCR, the gene was constitutively expressed in all tested organs/tissues, with higher level observed in blood, intestine and thymus. An induced expression of IFN-gamma at mRNA level was observed in peripheral blood leucocytes (PBLs) in response to in vitro stimulation of LPS and PolyI:C. The overexpression of IFN-gamma in the Chinese soft-shelled turtle artery (STA) cell line resulted in the increase in the expression of transcriptional regulators, such as IRF1, IRF7 and STAT1, and antiviral genes, such as Mx, PKR, implying possibly the existence of a conserved signalling network and role for IFN-gamma in the turtle. Furthermore, the infection of soft-shelled turtle iridovirus (STIV) in the cell line transfected with IFN-gamma may cause the cell death as demonstrated with the elevated lactate dehydrogenase (LDH) level and cell mortality. However, the mechanism involved in the antiviral activity may require further investigation. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Immunology ; Zoology
收录类别	SCI
资助信息	National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703]
原文出处	ELSEVIER SCI LTD
WOS记录号	WOS:000331479800012
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10468]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Fu, JP,Chen, SN,Zou, PF,et al. IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,43(1):87-95.
APA	Fu, JP.,Chen, SN.,Zou, PF.,Huang, B.,Guo, Z.,...&Nie, P.(2014).IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,43(1),87-95.
MLA	Fu, JP,et al."IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 43.1(2014):87-95.