A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature
	Yang, FF ; Xiang, WZ ; Sun, XM ; Wu, HL ; Li, T ; Long, LJ
刊名	MARINE DRUGS
	2014
卷号	12 期号:3 页码:1258-1270
关键词	lipid extraction wet microalga Picochlorum sp. ethanol biofuel
ISSN号	1660-3397
通讯作者	ycuyang@163.com ; xwz@scsio.ac.cn ; sunxm@scsio.ac.cn ; hlwu@scsio.ac.cn ; taoli@scsio.ac.cn ; longlj@scsio.ac.cn
中文摘要	A novel method using ethanol was proposed for extracting lipids from wet microalga Picochlorum sp. at room temperature and pressure. In this study, Central Composite design (CCD) was applied to investigate the optimum conditions of lipid extraction. The results revealed that the solvent to biomass ratio had the largest effect on lipid extraction efficiency, followed by extraction time and temperature. A high lipid extraction yield (33.04% of the dry weight) was obtained under the following extraction conditions: 5 mL solvents per gram of wet biomass for 37 min with gentle stirring at room temperature. The extraction yield was comparable to that obtained by the widely used Bligh-Dyer method. Furthermore, no significant differences in the distribution of lipid classes and fatty acid composition were observed according to different extraction methods. In conclusion, these results indicated that the proposed procedure using ethanol could extract lipids from wet biomass efficiently and had giant potential for lipid extraction at large scale.
学科主题	Pharmacology & Pharmacy
收录类别	SCI
资助信息	Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]
原文出处	MDPI AG
WOS记录号	WOS:000335752800006
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10463]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Yang, FF,Xiang, WZ,Sun, XM,et al. A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature[J]. MARINE DRUGS,2014,12(3):1258-1270.
APA	Yang, FF,Xiang, WZ,Sun, XM,Wu, HL,Li, T,&Long, LJ.(2014).A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature.MARINE DRUGS,12(3),1258-1270.
MLA	Yang, FF,et al."A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature".MARINE DRUGS 12.3(2014):1258-1270.