Mechanistic Insights into Polycycle Formation by Reductive Cyclization in Ikarugamycin Biosynthesis

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	Mechanistic Insights into Polycycle Formation by Reductive Cyclization in Ikarugamycin Biosynthesis
	Zhang, GT ; Zhang, WJ ; Zhang, QB ; Shi, T ; Ma, L ; Zhu, YG ; Li, SM ; Zhang, HB ; Zhao, YL ; Shi, R ; Zhang, CS
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2014
卷号	53 期号:19 页码:4840-4844
关键词	biosynthesis heterologous expression ikarugamycin polycyclic tetramate macrolactams reductive cyclization
ISSN号	1433-7851
通讯作者	czhang2006@gmail.com
中文摘要	Ikarugamycin is a member of the polycyclic tetramate macrolactams (PTMs) family of natural products with diverse biological activities. The biochemical mechanisms for the formation of polycyclic ring systems in PTMs remain elusive. The enzymatic mechanism of constructing an inner five-membered ring in ikarugamycin is reported. A three-gene-cassette ikaABC from the marine-derived Streptomyces sp. ZJ306 is sufficient for conferring ikarugamycin production in a heterologous host. IkaC catalyzes a reductive cyclization reaction to form the inner five-membered ring by a Michael addition-like reaction. This study provides the first biochemical evidence for polycycle formation in PTMs and suggests a reductive cyclization strategy which may be potentially applicable in general to the corresponding ring formation in other PTMs.
学科主题	Chemistry
收录类别	SCI
资助信息	National Natural Science Foundation of China [31125001, 21377085]; Ministry of Science and Technology of China [2010CB833805, 2012AA092104]; Chinese Academy of Sciences [XDA11030403]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-001, GD2012-D01-002]
原文出处	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000335202700013
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10440]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Zhang, GT,Zhang, WJ,Zhang, QB,et al. Mechanistic Insights into Polycycle Formation by Reductive Cyclization in Ikarugamycin Biosynthesis[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2014,53(19):4840-4844.
APA	Zhang, GT.,Zhang, WJ.,Zhang, QB.,Shi, T.,Ma, L.,...&Zhang, CS.(2014).Mechanistic Insights into Polycycle Formation by Reductive Cyclization in Ikarugamycin Biosynthesis.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,53(19),4840-4844.
MLA	Zhang, GT,et al."Mechanistic Insights into Polycycle Formation by Reductive Cyclization in Ikarugamycin Biosynthesis".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 53.19(2014):4840-4844.