Characterization of TCHQ-induced genotoxicity and mutagenesis using the pSP189 shuttle vector in mammalian cells

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Characterization of TCHQ-induced genotoxicity and mutagenesis using the pSP189 shuttle vector in mammalian cells
	Wang, Jing ; Yu, Shouyi ; Jiao, Shouhai ; Lv, Xiaowen ; Ma, Min ; Zhu, Ben-zhan ; Du, Yuguo
刊名	Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology
	2012
卷号	729 期号:1 页码:16-23
关键词	TCHQ Genotoxicity DNA mutation pSP189 Shuttle vector A549 cells
ISSN号	0027-5107
中文摘要	Tetrachlorohydroquinone (TCHQ) is a major toxic metabolite of the widely used wood preservative, pentachlorophenol (PCP), and it has also been implicated in PCP genotoxicity. However, the underlying mechanisms of genotoxicity and mutagenesis induced by TCHQ remain unclear. In this study, we examined the genotoxicity of TCHQ by using comet assays to detect DNA breakage and formation of TCHQ-DNA adducts. Then, we further verified the levels of mutagenesis by using the pSP189 shuttle vector in A549 human lung carcinoma cells. We demonstrated that TCHQ causes significant genotoxicity by inducing DNA breakage and forming DNA adducts. Additionally, DNA sequence analysis of the TCHQ-induced mutations revealed that 85.36% were single base substitutions, 9.76% were single base insertions, and 4.88% were large fragment deletions. More than 80% of the base substitutions occurred at G:C base pairs, and the mutations were G:C to C:G, G:C to T:A or G:C to A:T transversions and transitions. The most common types of mutations in A549 cells were G:C to A:T (37.14%) and A:T to C:G transitions (14.29%) and G:C to C:G (34.29%) and G:C to T:A (11.43%) transversions. We identified hotspots at nucleotides 129, 141, and 155 in the supF gene of plasmid pSP189. These mutation hotspots accounted for 63% of all single base substitutions. We conclude that TCHQ induces sequence-specific DNA mutations at high frequencies. Therefore, the safety of using this product would be carefully examined. (C) 2011 Elsevier B.V. All rights reserved.
WOS记录号	WOS:000299980900002
公开日期	2014-11-27
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/8035]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Wang, Jing,Yu, Shouyi,Jiao, Shouhai,et al. Characterization of TCHQ-induced genotoxicity and mutagenesis using the pSP189 shuttle vector in mammalian cells[J]. Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology,2012,729(1):16-23.
APA	Wang, Jing.,Yu, Shouyi.,Jiao, Shouhai.,Lv, Xiaowen.,Ma, Min.,...&Du, Yuguo.(2012).Characterization of TCHQ-induced genotoxicity and mutagenesis using the pSP189 shuttle vector in mammalian cells.Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology,729(1),16-23.
MLA	Wang, Jing,et al."Characterization of TCHQ-induced genotoxicity and mutagenesis using the pSP189 shuttle vector in mammalian cells".Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology 729.1(2012):16-23.