Rapid lipid profiling of bacteria by online MALDI-TOF mass spectrometry

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 大气环境科学实验室

	Rapid lipid profiling of bacteria by online MALDI-TOF mass spectrometry
	Shu, Xi ; Li, Yueyan ; Liang, Miao ; Yang, Bo ; Liu, Changgeng ; Wang, Youfeng ; Shu, Jinian
刊名	Physics; Spectroscopy
	2012
卷号	321 期号:1 页码:71-76
关键词	Lipid Online MALDI Bacterium Escherichia coli Salmonella Bacillus subtilis
ISSN号	1387-3806
中文摘要	The rapid identification of bacteria is of increasing interest in food, biosafety, and clinical analysis. In this paper, we report a technique for rapid lipid profiling of bacteria by online droplet-deposited MALDI mass spectrometry. In the experiment, lipids in bacterial cells (Escherichia coli, Salmonella, and Bacillus subtilis) are extracted with the mixture of chloroform/methanol via ultrasonication. The extracts are nebulized into suspended droplets and then instantly analyzed with an online droplet-deposited MALDI-TOF mass spectrometer. The lipid profiles of the three bacteria cultured in nutrient broth and LB (Lysogeny Broth) medium are obtained. The lipids phosphatidylethanolamine (PE), diglycosyl diacylglycerol (DGDG), and phosphatidylglycerol (PG) are observed in the MALDI-TOF mass spectra. The MALDI-TOF mass spectrum of each bacterial extract shows a characteristic profile. The experimental results demonstrate that the present technique is capable of conducting rapid lipid analysis and has the potential for rapid bioaerosol detection. (c) 2012 Elsevier B.V. All rights reserved.
WOS记录号	WOS:000307691500010
公开日期	2014-11-26
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/8020]
专题	生态环境研究中心_大气环境科学实验室
推荐引用方式 GB/T 7714	Shu, Xi,Li, Yueyan,Liang, Miao,et al. Rapid lipid profiling of bacteria by online MALDI-TOF mass spectrometry[J]. Physics; Spectroscopy,2012,321(1):71-76.
APA	Shu, Xi.,Li, Yueyan.,Liang, Miao.,Yang, Bo.,Liu, Changgeng.,...&Shu, Jinian.(2012).Rapid lipid profiling of bacteria by online MALDI-TOF mass spectrometry.Physics; Spectroscopy,321(1),71-76.
MLA	Shu, Xi,et al."Rapid lipid profiling of bacteria by online MALDI-TOF mass spectrometry".Physics; Spectroscopy 321.1(2012):71-76.