Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column

	Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column
	Li, Xiaolong 1,2,3; Guo, Zhimou 1; Wang, Chaoran 1; Shen, Aijin 1; Liu, Yanfang 1; Zhang, Xiuli 1; Zhao, Weijie 2; Liang, Xinmiao 1
刊名	journal of pharmaceutical and biomedical analysis
	2014-04-15
卷号	92 页码:105-113
关键词	Purification Bufadienolides Bufo bufo gargarizans Cantor Reversed-phase liquid chromatography Positively charged C18
通讯作者	刘艳芳 ; 梁鑫淼
产权排序	待补充
合作状况	英
英文摘要	as a kind of promising anticancer compounds, the preparation of bufadienolides is a hot study spot. however, due to the complexity of biological sample, the purification of bufadienolides from a crude sample (toad skin) is a tough work. in this paper, we reported a new way based on positively charged c18 material (xcharge c18) to quickly separate and purify bufadienolides from toad skin. by this method, the different ionic feature of the amino acid conjugated bufadienolides (aacbs) and the free form bufadienolides (aaubs) was firstly utilized to obtain distinct separation selectivity on the xcharge c18 column. additionally, the peak tailing problem of aacbs on conventional c18 was resolved and better resolutions were achieved on the xcharge c18, thus, two kinds of bufadienolides on one column were successfully purified respectively. taking f13 as an example, the method was validated by liquid chromatography-mass spectrometry (lc-ms), and then 4 aacbs as well as 4 aaubs were simultaneously purified by preparative xcharge c18. in addition, the application of this method in other fractions was also validated. the results suggested that the developed method is a practical and promising tool for efficient separation and purification of bufadienolides from toad skin. (c) 2014 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences ; life sciences & biomedicine
类目[WOS]	chemistry, analytical ; pharmacology & pharmacy
研究领域[WOS]	chemistry ; pharmacology & pharmacy
关键词[WOS]	flight mass-spectrometry ; drug chan-su ; liquid-chromatography ; cinobufacini injection ; toad skin ; steroids ; separation ; medicine ; biotransformation ; cinobufotalin
收录类别	SCI
资助信息	1，1
原文出处	113
语种	英语
WOS记录号	WOS:000335487300015
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119810]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Univ Technol, State Key Lab Fine Chem, Sch Pharmaceut Sci & Technol, Dalian 116024, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiaolong,Guo, Zhimou,Wang, Chaoran,et al. Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column[J]. journal of pharmaceutical and biomedical analysis,2014,92:105-113.
APA	Li, Xiaolong.,Guo, Zhimou.,Wang, Chaoran.,Shen, Aijin.,Liu, Yanfang.,...&Liang, Xinmiao.(2014).Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column.journal of pharmaceutical and biomedical analysis,92,105-113.
MLA	Li, Xiaolong,et al."Purification of bufadienolides from the skin of Bufo bufo gargarizans Cantor with positively charged C18 column".journal of pharmaceutical and biomedical analysis 92(2014):105-113.