Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip

	Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip
	Wen, Hui; Gao, Xinghua; Qin, Jianhua
刊名	integrative biology
	2014
卷号	6 期号:1 页码:35-43
ISSN号	1757-9694
通讯作者	秦建华
产权排序	待补充
合作状况	英
英文摘要	c. elegans is widely used as a model organism in the study of aging and evaluation of anti-aging drugs due to its unique characteristics. in this work, we set out to investigate polydatin, a natural resveratrol glycoside, and its role in extending lifespan, improving oxidative stress resistance, and the possible regulation mechanism involved in the insulin/igf-1 signaling (iis) pathway for the first time by using a flexible microfluidic device. the effects of polydatin on the lifespan, oxidative stress resistance, mobility and the expression of aging-related proteins and genes were explored. polydatin was found to significantly extend the mean lifespan of worms by up to 30.7% and 62.1% under normal and acute stress conditions respectively. it improved the expression of the inducible oxidative stress protein (gst-4) and corresponding stroke frequencies in the transgenic cl2166 strain. moreover, it also increased sod-3::gfp expression in cf1553 worms and promoted daf-16 nucleus translocation in tj356 worms. the longevity-extending role of polydatin is partly attributed to its anti-oxidative activity and increased oxidative stress resistance by regulating the stress-resistance related proteins sod-3, and daf-16 expression at protein and mrna levels involved in the iis pathway. the established microfluidic platform is capable of flexible operation with multiple functions, which not only supports the individual worm's long-term culture with sufficient nutrient exchange, but also facilitates mobility monitoring of the worm, immobilizing and imaging in a controllable and parallel manner. these interesting findings reported here highlight the significance of the natural compound polydatin in the study of aging-related diseases, and the utility of the microfluidic platform for applications in aging studies.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	cell biology
研究领域[WOS]	cell biology
关键词[WOS]	life-span ; c-elegans ; resveratrol ; activation ; diseases ; protein ; stress ; injury ; piceid ; genes
收录类别	SCI
资助信息	1，1
原文出处	43
语种	英语
WOS记录号	WOS:000328885900004
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119696]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Biotechnol Lab, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wen, Hui,Gao, Xinghua,Qin, Jianhua. Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip[J]. integrative biology,2014,6(1):35-43.
APA	Wen, Hui,Gao, Xinghua,&Qin, Jianhua.(2014).Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip.integrative biology,6(1),35-43.
MLA	Wen, Hui,et al."Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip".integrative biology 6.1(2014):35-43.