Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase

	Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase
	Long, Zhen; Guo, Zhimou; Xue, Xingya; Zhang, Xiuli; Nordahl, Lilly; Liang, Xinmiao
刊名	analytica chimica acta
	2013-12-04
卷号	804 页码:304-312
关键词	Strong cation exchange Highly hydrophilic bases Purification Natural product Symmetrical peak shape
通讯作者	张秀莉 ; 梁鑫淼
产权排序	待补充
合作状况	英
英文摘要	compared to moderately and weakly hydrophilic bases, highly polar basic compounds are even more difficult to separate due to their poor retention in reversed phase (rp) mode. this study described the successful applications of a strong cation exchange (scx) stationary phase to achieve symmetric peak shape, adequate retention and selectivity in the separation of very polar basic compounds. salt and acetonitrile concentrations were adjusted to optimize the separation. good correlations (r-2 = 0.998-1.000) between the logarithm of the retention factor and the logarithm of salt or acetonitrile concentration were obtained. gradients generated by changing salt or acetonitrile concentration were compared for the analysis of different highly polar bases. although all of the analytes were eluted more quickly with an acetonitrile gradient, the effect of the gradients tested on peak width and peak shape varied with respect to analyte. in addition, the effects of different types of cation and anion additives were also investigated. after separation parameters were acquired, the scx-based method was utilized to analyze highly hydrophilic alkaloids from scopolia tangutica maxim with high separation efficiency (plate numbers >32,000 m(-1)). concurrently, one very polar alkaloid fraction was purified with symmetric peak shape using the current method. our results suggest that scx stationary phase can be used as an alternative to rp stationary phase in the analysis and purification of highly hydrophilic basic compounds. (c) 2013 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	performance liquid-chromatography ; reversed-phase ; retention behavior ; peak shape ; drugs ; hplc ; identification ; enrichment ; additives ; serotonin
收录类别	SCI
资助信息	1，1
原文出处	312
语种	英语
WOS记录号	WOS:000327493500040
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119531]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Dalian Univ Technol, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Long, Zhen,Guo, Zhimou,Xue, Xingya,et al. Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase[J]. analytica chimica acta,2013,804:304-312.
APA	Long, Zhen,Guo, Zhimou,Xue, Xingya,Zhang, Xiuli,Nordahl, Lilly,&Liang, Xinmiao.(2013).Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase.analytica chimica acta,804,304-312.
MLA	Long, Zhen,et al."Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase".analytica chimica acta 804(2013):304-312.