FTO-mediated formation of N-6-hydroxymethyladenosine and N-6-formyladenosine in mammalian RNA

	FTO-mediated formation of N-6-hydroxymethyladenosine and N-6-formyladenosine in mammalian RNA
	Fu, Ye 1,2; Jia, Guifang 1,2; Pang, Xueqin 3,4,5,6; Wang, Richard N.1,2; Wang, Xiao 1,2; Li, Charles J.1,2; Smemo, Scott 7; Dai, Qing 1,2; Bailey, Kathleen A.7; Nobrega, Marcelo A.7
刊名	nature communications
	2013-04-01
卷号	4 页码:1798
通讯作者	ye fu
产权排序	待补充
合作状况	英
英文摘要	n-6-methyladenosine is a prevalent internal modification in messenger rna and non-coding rna affecting various cellular pathways. here we report the discovery of two additional modifications, n-6-hydroxymethyladenosine (hm(6)a) and n-6-formyladenosine (f(6)a), in mammalian messenger rna. we show that fe-ii-and alpha-ketoglutarate-dependent fat mass and obesity-associated (fto) protein oxidize n-6-methyladenosine to generate n-6-hydroxymethyladenosine as an intermediate modification, and n-6-formyladenosine as a further oxidized product. n-6-hydroxymethyladenosine and n-6-formyladenosine have half-life times of similar to 3 h in aqueous solution under physiological relevant conditions, and are present in isolated messenger rna from human cells as well as mouse tissues. these previously unknown modifications derived from the prevalent n-6-methyladenosine in messenger rna, formed through oxidative rna demethylation, may dynamically modulate rna-protein interactions to affect gene expression regulation.
学科主题	物理化学
WOS标题词	science & technology
类目[WOS]	multidisciplinary sciences
研究领域[WOS]	science & technology - other topics
关键词[WOS]	obesity-associated fto ; messenger-rna ; escherichia-coli ; oxidative demethylation ; adult obesity ; dna ; gene ; alkb ; 5-hydroxymethylcytosine ; 5-carboxylcytosine
收录类别	SCI
资助信息	3，3
原文出处	1798
语种	英语
WOS记录号	WOS:000318872100155
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119390]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Univ Chicago, Dept Chem, Chicago, IL 60637 USA 2.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA 3.Univ Wisconsin, Dept Chem, Madison, WI 53706 USA 4.Univ Wisconsin, Inst Theoret Chem, Madison, WI 53706 USA 5.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Liaoning, Peoples R China 6.Chinese Acad Sci, Dalian Inst Chem Phys, Ctr Theoret & Computat Chem, Dalian 116023, Liaoning, Peoples R China 7.Univ Chicago, Dept Human Genet, Chicago, IL 60637 USA
推荐引用方式 GB/T 7714	Fu, Ye,Jia, Guifang,Pang, Xueqin,et al. FTO-mediated formation of N-6-hydroxymethyladenosine and N-6-formyladenosine in mammalian RNA[J]. nature communications,2013,4:1798.
APA	Fu, Ye.,Jia, Guifang.,Pang, Xueqin.,Wang, Richard N..,Wang, Xiao.,...&He, Chuan.(2013).FTO-mediated formation of N-6-hydroxymethyladenosine and N-6-formyladenosine in mammalian RNA.nature communications,4,1798.
MLA	Fu, Ye,et al."FTO-mediated formation of N-6-hydroxymethyladenosine and N-6-formyladenosine in mammalian RNA".nature communications 4(2013):1798.