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	Immune responses to vaccines involving a combined antigen-nanoparticle mixture and nanoparticle-encapsulated antigen formulation
	Zhang, Weifeng1,2; Wang, Lianyan1; Liu, Yuan1,2; Chen, Xiaoming1,2; Liu, Qi1,2; Jia, Jilei1,2; Yang, Tingyuan1; Qiu, Shaohui3; Ma, Guanghui1
刊名	BIOMATERIALS
	2014-07-01
卷号	35期号:23页码:6086-6097
关键词	Adjuvant Nanoparticles Vaccine formulations Immune response Mechanism of action
ISSN号	0142-9612
其他题名	Biomaterials
中文摘要	Many physicochemical characteristics significantly influence the adjuvant effect of micro/nanoparticles; one critical factor is the kinetics of antigen exposure to the immune system by particle-adjuvanted vaccines. Here, we investigated how various antigen-nanoparticle formulations impacted antigen exposure to the immune system and the resultant antigen-specific immune responses. We formulated antigen with poly(lactic-co-glycolic acid) (PLGA) nanoparticles by encapsulating antigen within nanoparticles or by simply mixing soluble antigen with the nanoparticles. Our results indicated that the combined formulation (composed of antigen encapsulated in nanoparticles and antigen mixed with nanoparticles) induced more powerful antigen-specific immune responses than each single-component formulation. Mice immunized with the combined vaccine formulation displayed enhanced induction of antigen-specific IgG antibodies with high avidity, increased cytokine secretion by splenocytes, and improved generation of memory T cell. Enhanced immune responses elicited by the combined vaccine formulation might be attributed to the antigen-depot effect at the injection site, effective provision of both adequate initial antigen exposure and long-term antigen persistence, and efficient induction of dendritic cell (DC) activation and follicular helper T cell differentiation in draining lymph nodes. Understanding the effect of antigen nanoparticle formulations on the resultant immune responses might have significant implications for rational vaccine design. (C) 2014 Elsevier Ltd. All rights reserved.
英文摘要	Many physicochemical characteristics significantly influence the adjuvant effect of micro/nanoparticles; one critical factor is the kinetics of antigen exposure to the immune system by particle-adjuvanted vaccines. Here, we investigated how various antigen-nanoparticle formulations impacted antigen exposure to the immune system and the resultant antigen-specific immune responses. We formulated antigen with poly(lactic-co-glycolic acid) (PLGA) nanoparticles by encapsulating antigen within nanoparticles or by simply mixing soluble antigen with the nanoparticles. Our results indicated that the combined formulation (composed of antigen encapsulated in nanoparticles and antigen mixed with nanoparticles) induced more powerful antigen-specific immune responses than each single-component formulation. Mice immunized with the combined vaccine formulation displayed enhanced induction of antigen-specific IgG antibodies with high avidity, increased cytokine secretion by splenocytes, and improved generation of memory T cell. Enhanced immune responses elicited by the combined vaccine formulation might be attributed to the antigen-depot effect at the injection site, effective provision of both adequate initial antigen exposure and long-term antigen persistence, and efficient induction of dendritic cell (DC) activation and follicular helper T cell differentiation in draining lymph nodes. Understanding the effect of antigen nanoparticle formulations on the resultant immune responses might have significant implications for rational vaccine design. (C) 2014 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]	Engineering ; Materials Science
关键词[WOS]	T-CELL RESPONSE ; PARTICLE-SIZE ; ADJUVANTS ; DELIVERY ; MICROPARTICLES ; KINETICS ; PROMOTE ; DIFFERENTIATION ; HYDROPHOBICITY ; PERSISTENCE
收录类别	SCI
原文出处	://WOS:000337212200011
语种	英语
WOS记录号	WOS:000337212200011
公开日期	2014-08-28
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/11036]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Inst Food & Drug Control, Beijing 100050, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Weifeng,Wang, Lianyan,Liu, Yuan,et al. Immune responses to vaccines involving a combined antigen-nanoparticle mixture and nanoparticle-encapsulated antigen formulation[J]. BIOMATERIALS,2014,35(23):6086-6097.
APA	Zhang, Weifeng.,Wang, Lianyan.,Liu, Yuan.,Chen, Xiaoming.,Liu, Qi.,...&Ma, Guanghui.(2014).Immune responses to vaccines involving a combined antigen-nanoparticle mixture and nanoparticle-encapsulated antigen formulation.BIOMATERIALS,35(23),6086-6097.
MLA	Zhang, Weifeng,et al."Immune responses to vaccines involving a combined antigen-nanoparticle mixture and nanoparticle-encapsulated antigen formulation".BIOMATERIALS 35.23(2014):6086-6097.

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