Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp C2

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	Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp C2
	Chen, Hui 1; Zhang, Yunming 1,2; He, Chenliu 1; Wang, Qiang 1
刊名	PLANT AND CELL PHYSIOLOGY
	2014-03-01
卷号	55 期号:3 页码:634-644
关键词	Ca2+ channel Ca2+ signal transduction Chlorella sp C2 Neutral lipid synthesis Nitrogen starvation
ISSN号	0032-0781
通讯作者	Wang, Q (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China.
中文摘要	Changes in the cytosolic Ca2+ levels and the role of Ca2+ signal transduction in neutral lipid synthesis in Chlorella sp. C2 under nitrogen starvation conditions were investigated. The results detected by using the scanning ion-selective electrode technique demonstrate that nitrogen starvation induced significant Ca2+ influx across the plasma membrane into cells. Ca2+ fluorescence imaging and flow cytometry were used to estimate the effect of this Ca2+ influx on the generation of the Ca2+ signal, and the results showed that the cytosolic Ca2+ concentration increased transiently and then remained at a stable, high level when the cells were exposed to nitrogen starvation. However, the increase could be inhibited by pre-treatment with the Ca2+ channel blockers ruthenium red, verapamil and GdCl3, indicating that both the influx of Ca2+ from the extracellular space via Ca2+ channels that are localized in the plasma membrane and the release of Ca2+ from intracellular calcium storage via the internal calcium store were required for the generation and transduction of the Ca2+ signal. During nitrogen starvation, neutral lipid synthesis in Chlorella sp. C2 in response to stress conditions was also inhibited to differing degrees by pre-treatment with the three Ca2+ channel blockers, demonstrating the regulation of Ca2+ via these Ca2+ channels in neutral lipid synthesis. The results suggested that by transduction of extracellular stress signals into the cell and the regulation of the Ca2+ signal in neutral lipid synthesis, Ca2+ signal transduction played important roles in the response mechanism of Chlorella sp. C2 to nitrogen starvation.
英文摘要	Changes in the cytosolic Ca2+ levels and the role of Ca2+ signal transduction in neutral lipid synthesis in Chlorella sp. C2 under nitrogen starvation conditions were investigated. The results detected by using the scanning ion-selective electrode technique demonstrate that nitrogen starvation induced significant Ca2+ influx across the plasma membrane into cells. Ca2+ fluorescence imaging and flow cytometry were used to estimate the effect of this Ca2+ influx on the generation of the Ca2+ signal, and the results showed that the cytosolic Ca2+ concentration increased transiently and then remained at a stable, high level when the cells were exposed to nitrogen starvation. However, the increase could be inhibited by pre-treatment with the Ca2+ channel blockers ruthenium red, verapamil and GdCl3, indicating that both the influx of Ca2+ from the extracellular space via Ca2+ channels that are localized in the plasma membrane and the release of Ca2+ from intracellular calcium storage via the internal calcium store were required for the generation and transduction of the Ca2+ signal. During nitrogen starvation, neutral lipid synthesis in Chlorella sp. C2 in response to stress conditions was also inhibited to differing degrees by pre-treatment with the three Ca2+ channel blockers, demonstrating the regulation of Ca2+ via these Ca2+ channels in neutral lipid synthesis. The results suggested that by transduction of extracellular stress signals into the cell and the regulation of the Ca2+ signal in neutral lipid synthesis, Ca2+ signal transduction played important roles in the response mechanism of Chlorella sp. C2 to nitrogen starvation.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences ; Cell Biology
研究领域[WOS]	Plant Sciences ; Cell Biology
关键词[WOS]	CROSS-TALK ; CALCIUM ; EXPRESSION ; STRESS ; PLANTS ; PURIFICATION ; SPECIFICITY ; MICROALGAE ; TRANSPORT ; REDUCTASE
收录类别	SCI
资助信息	National Program on Key Basic Research Project [2011CB200902]; National Natural Science Foundation of China [31300030]
语种	英语
WOS记录号	WOS:000333096200014
公开日期	2014-08-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20110]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100094, Peoples R China
推荐引用方式 GB/T 7714	Chen, Hui,Zhang, Yunming,He, Chenliu,et al. Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp C2[J]. PLANT AND CELL PHYSIOLOGY,2014,55(3):634-644.
APA	Chen, Hui,Zhang, Yunming,He, Chenliu,&Wang, Qiang.(2014).Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp C2.PLANT AND CELL PHYSIOLOGY,55(3),634-644.
MLA	Chen, Hui,et al."Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp C2".PLANT AND CELL PHYSIOLOGY 55.3(2014):634-644.