Complementary DNA sequences of the constant regions of T-cell antigen receptors alpha, beta and gamma in mandarin fish, Siniperca chuatsi Basilewsky, and their transcriptional changes after stimulation with Flavobacterium columnare | |
Tian, J. Y.1; Qi, Z. T.2; Wu, N.3; Chang, M. X.4; Nie, P.4 | |
刊名 | JOURNAL OF FISH DISEASES
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2014-02-01 | |
卷号 | 37期号:2页码:89-101 |
关键词 | TCR Siniperca chuatsi mandarin fish Flavobacterium columnare |
ISSN号 | 0140-7775 |
通讯作者 | Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China. |
中文摘要 | In this study, the constant-region genes (C alpha, C beta and C gamma) that encode the T-cell antigen receptor (TCR) alpha, beta and gamma chains were cloned from mandarin fish, Siniperca chuatsi Basilewsky, an important freshwater fish species in China. The complementary DNA sequences of C alpha, C beta and C gamma were 843, 716 and 906base pairs (bp) in length and had a 465-, 289- and 360-bp 3 ' untranslated region, encoding 125, 142 and 182 amino acids, respectively. The amino-acid sequences of the constant regions of mandarin fish TCR alpha, beta and gamma chains (encoded by C alpha, C beta and C gamma, respectively) were most similar to those of their teleost counterparts, showing 60% similarity with pufferfish, 48% similarity with Atlantic salmon and 57% similarity with flounder, respectively. The phylogenetic analysis revealed that the mandarin fish C alpha, C beta and C gamma were clustered, respectively, with their vertebrate counterparts. The mandarin fish C alpha, C beta and C gamma could also be separated into four domains: immunoglobulin; connecting peptide (CP); transmembrane (TM); and cytoplasmic tail. Several conserved features in mammalian TCRs were also found in those of mandarin fish, such as a conserved cysteine residue in the CP domain of C alpha, necessary for creating an interchain disulphide bond with the TCR beta chain, and a conserved antigen receptor TM motif in C alpha and C beta. Meanwhile, transcripts of C alpha, C beta and C gamma were detectable in all examined organs, with a stronger signal observed in lymphoid organs. In addition, the temporal transcriptional changes for C alpha and C gamma were investigated, 1, 2, 3, 4, 5, 6 and 8weeks after stimulation with Flavobacterium columnare, in head kidney, spleen, blood, thymus, gill and intestine, using real-time polymerase chain reaction. The results demonstrated stimulation-dependent up-regulations in almost all tissues examined, which indicates that T cells may play important roles in preventing mandarin fish from bacterial invasion. In particular, apart from thymus, T cells were distributed mainly in gill and intestine, where striking up-regulation of C gamma was also observed. These results will facilitate functional studies of teleost TCRs and T cells. |
英文摘要 | In this study, the constant-region genes (C alpha, C beta and C gamma) that encode the T-cell antigen receptor (TCR) alpha, beta and gamma chains were cloned from mandarin fish, Siniperca chuatsi Basilewsky, an important freshwater fish species in China. The complementary DNA sequences of C alpha, C beta and C gamma were 843, 716 and 906base pairs (bp) in length and had a 465-, 289- and 360-bp 3 ' untranslated region, encoding 125, 142 and 182 amino acids, respectively. The amino-acid sequences of the constant regions of mandarin fish TCR alpha, beta and gamma chains (encoded by C alpha, C beta and C gamma, respectively) were most similar to those of their teleost counterparts, showing 60% similarity with pufferfish, 48% similarity with Atlantic salmon and 57% similarity with flounder, respectively. The phylogenetic analysis revealed that the mandarin fish C alpha, C beta and C gamma were clustered, respectively, with their vertebrate counterparts. The mandarin fish C alpha, C beta and C gamma could also be separated into four domains: immunoglobulin; connecting peptide (CP); transmembrane (TM); and cytoplasmic tail. Several conserved features in mammalian TCRs were also found in those of mandarin fish, such as a conserved cysteine residue in the CP domain of C alpha, necessary for creating an interchain disulphide bond with the TCR beta chain, and a conserved antigen receptor TM motif in C alpha and C beta. Meanwhile, transcripts of C alpha, C beta and C gamma were detectable in all examined organs, with a stronger signal observed in lymphoid organs. In addition, the temporal transcriptional changes for C alpha and C gamma were investigated, 1, 2, 3, 4, 5, 6 and 8weeks after stimulation with Flavobacterium columnare, in head kidney, spleen, blood, thymus, gill and intestine, using real-time polymerase chain reaction. The results demonstrated stimulation-dependent up-regulations in almost all tissues examined, which indicates that T cells may play important roles in preventing mandarin fish from bacterial invasion. In particular, apart from thymus, T cells were distributed mainly in gill and intestine, where striking up-regulation of C gamma was also observed. These results will facilitate functional studies of teleost TCRs and T cells. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
研究领域[WOS] | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
关键词[WOS] | TELEOST FISH ; TCR ALPHA ; RAINBOW-TROUT ; AEROMONAS-HYDROPHILA ; GENOMIC ORGANIZATION ; MOLECULAR-CLONING ; IMMUNE-RESPONSE ; SPARUS-AURATA ; COMMON CARP ; DELTA CELLS |
收录类别 | SCI |
资助信息 | National Natural Science Foundation of China (NSFC) [U0631010]; People's Government of Guangdong Province; NSFC [31230075]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [10KJB24000, 10KJB24001] |
语种 | 英语 |
WOS记录号 | WOS:000334388300002 |
公开日期 | 2014-08-08 |
内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/20050] ![]() |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
作者单位 | 1.Natl Oceanog Ctr, Qingdao, Shandong, Peoples R China 2.Yancheng Inst Technol, Key Lab Aquaculture & Ecol Coastal Pool Jiangsu P, Yancheng, Jiangsu, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Mol Immunol, Wuhan 430072, Hubei Province, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China |
推荐引用方式 GB/T 7714 | Tian, J. Y.,Qi, Z. T.,Wu, N.,et al. Complementary DNA sequences of the constant regions of T-cell antigen receptors alpha, beta and gamma in mandarin fish, Siniperca chuatsi Basilewsky, and their transcriptional changes after stimulation with Flavobacterium columnare[J]. JOURNAL OF FISH DISEASES,2014,37(2):89-101. |
APA | Tian, J. Y.,Qi, Z. T.,Wu, N.,Chang, M. X.,&Nie, P..(2014).Complementary DNA sequences of the constant regions of T-cell antigen receptors alpha, beta and gamma in mandarin fish, Siniperca chuatsi Basilewsky, and their transcriptional changes after stimulation with Flavobacterium columnare.JOURNAL OF FISH DISEASES,37(2),89-101. |
MLA | Tian, J. Y.,et al."Complementary DNA sequences of the constant regions of T-cell antigen receptors alpha, beta and gamma in mandarin fish, Siniperca chuatsi Basilewsky, and their transcriptional changes after stimulation with Flavobacterium columnare".JOURNAL OF FISH DISEASES 37.2(2014):89-101. |
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