Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis

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	Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis
	Zhong, J-X; Zhou, L.; Li, Z.; Gui, J-F
刊名	CELL DEATH AND DIFFERENTIATION
	2014-06-01
卷号	21 期号:6 页码:1013-1024
关键词	Noxa Bik Wnt4b Mcl1 mitosis apoptosis
ISSN号	1350-9047
通讯作者	Gui, JF (reprint author), Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
中文摘要	Noxa functions in apoptosis and immune system of vertebrates, but its activities in embryo development remain unclear. In this study, we have studied the role of zebrafish Noxa (zNoxa) by using zNoxa-specifc morpholino knockdown and overexpression approaches in developing zebrafish embryos. Expression pattern analysis indicates that zNoxa transcript is of maternal origin, which displays a uniform distribution in early embryonic development until shield stage, and the zygote zNoxa transcription is initiated from this stage and mainly localized in YSL of the embryos. The zNoxa expression alterations result in strong embryonic development defects, demonstrating that zNoxa regulates apoptosis from 75% epiboly stage of development onward, in which zNoxa firstly induces the expression of zBik, and then cooperates with zBik to regulate apoptosis. Moreover, zNoxa knockdown also causes a reduction in number of mitotic cells before 8 h.p.f., suggesting that zNoxa also promotes mitosis before 75% epiboly stage. The effect of zNoxa on mitosis is mediated by zWnt4b in early embryos, whereas zMcl1a and zMcl1b suppress the ability of zNoxa to regulate mitosis and apoptosis at different developmental stages. In addition, mammalian mouse Noxa (mNoxa) mRNA was demonstrated to rescue the arrest of mitosis when zNoxa was knocked down, suggesting that mouse and zebrafish Noxa might have similar dual functions. Therefore, the current findings indicate that Noxa is a novel regulator of early mitosis before 75% epiboly stage when it translates into a key mediator of apoptosis in subsequent embryogenesis.
英文摘要	Noxa functions in apoptosis and immune system of vertebrates, but its activities in embryo development remain unclear. In this study, we have studied the role of zebrafish Noxa (zNoxa) by using zNoxa-specifc morpholino knockdown and overexpression approaches in developing zebrafish embryos. Expression pattern analysis indicates that zNoxa transcript is of maternal origin, which displays a uniform distribution in early embryonic development until shield stage, and the zygote zNoxa transcription is initiated from this stage and mainly localized in YSL of the embryos. The zNoxa expression alterations result in strong embryonic development defects, demonstrating that zNoxa regulates apoptosis from 75% epiboly stage of development onward, in which zNoxa firstly induces the expression of zBik, and then cooperates with zBik to regulate apoptosis. Moreover, zNoxa knockdown also causes a reduction in number of mitotic cells before 8 h.p.f., suggesting that zNoxa also promotes mitosis before 75% epiboly stage. The effect of zNoxa on mitosis is mediated by zWnt4b in early embryos, whereas zMcl1a and zMcl1b suppress the ability of zNoxa to regulate mitosis and apoptosis at different developmental stages. In addition, mammalian mouse Noxa (mNoxa) mRNA was demonstrated to rescue the arrest of mitosis when zNoxa was knocked down, suggesting that mouse and zebrafish Noxa might have similar dual functions. Therefore, the current findings indicate that Noxa is a novel regulator of early mitosis before 75% epiboly stage when it translates into a key mediator of apoptosis in subsequent embryogenesis.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	DIFFERENTIAL EXPRESSION ; PARALICHTHYS-OLIVACEUS ; CARASSIUS-AURATUS ; MOLECULAR-CLONING ; SKELETAL-MUSCLE ; BCL-2 FAMILY ; SEX REVERSAL ; CELL-DEATH ; GENE ; PROTEINS
收录类别	SCI
资助信息	National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]
语种	英语
WOS记录号	WOS:000335966900015
公开日期	2014-08-08
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20039]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhong, J-X,Zhou, L.,Li, Z.,et al. Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis[J]. CELL DEATH AND DIFFERENTIATION,2014,21(6):1013-1024.
APA	Zhong, J-X,Zhou, L.,Li, Z.,&Gui, J-F.(2014).Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis.CELL DEATH AND DIFFERENTIATION,21(6),1013-1024.
MLA	Zhong, J-X,et al."Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis".CELL DEATH AND DIFFERENTIATION 21.6(2014):1013-1024.