cDNA cloning and expression of vitamin-K Epoxide Reductase (VKOR) gene from orange spotted grouper Epinephelus coioides

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	cDNA cloning and expression of vitamin-K Epoxide Reductase (VKOR) gene from orange spotted grouper Epinephelus coioides
	Citarasu, T.1,2; Michaelbabu, M.1; Zhou, L.2; Yang, W.2; Gui, J. F.2
刊名	INDIAN JOURNAL OF GEO-MARINE SCIENCES
	2013-09-01
卷号	42 期号:5 页码:573-579
关键词	Epinephelus coioides Vitamin-K Epoxide Reductase cDNA cloning
ISSN号	0379-5136
通讯作者	Citarasu, T (reprint author), Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Kanyakumari 629502, Tamil Nadu, India.
中文摘要	The cDNA sequence encoding Vitamin-K Epoxide Reductase (VKOR) was constructed from hypothalamus and pituitary cDNA libraries of Orange spotted Grouper Epinephelus coioides. VKOR gene contains 610 nucleotides including the open reading frame (ORE) of length 399 bp. ORE was started with ATG codon at the position of 199 and terminated with a stop codon TAA of 585. ORE sequence had 128 amino acids and the predicted protein molecular weight is approximately 14.08 kDa (GenBank: FJ939281.1). Expression of VKOR gene in different tissues was further examined using one-step RT-PCR. RT PCR result revealed that, VKOR mRNA expression was detected only in liver, kidney, hypothalamus and pituitary. VKOR gene sequence of E. coioides aligned with related sequences by multiple sequence alignment by clustal X and phylogenetic tree was generated by Neighbor Joining method using Genious v 5.4. E. coioides VKOR had the highest identity and homology with the puffer fish, Tetraodon nigroviridis, Takifugu rubripes and Zebra fish Danio rerio. Based on the VKOR expression in the kidney, liver hypothalamus and pituitary of the seven year's old (sex reversal stage) E. coioides, it may be involved in sex reverse.
英文摘要	The cDNA sequence encoding Vitamin-K Epoxide Reductase (VKOR) was constructed from hypothalamus and pituitary cDNA libraries of Orange spotted Grouper Epinephelus coioides. VKOR gene contains 610 nucleotides including the open reading frame (ORE) of length 399 bp. ORE was started with ATG codon at the position of 199 and terminated with a stop codon TAA of 585. ORE sequence had 128 amino acids and the predicted protein molecular weight is approximately 14.08 kDa (GenBank: FJ939281.1). Expression of VKOR gene in different tissues was further examined using one-step RT-PCR. RT PCR result revealed that, VKOR mRNA expression was detected only in liver, kidney, hypothalamus and pituitary. VKOR gene sequence of E. coioides aligned with related sequences by multiple sequence alignment by clustal X and phylogenetic tree was generated by Neighbor Joining method using Genious v 5.4. E. coioides VKOR had the highest identity and homology with the puffer fish, Tetraodon nigroviridis, Takifugu rubripes and Zebra fish Danio rerio. Based on the VKOR expression in the kidney, liver hypothalamus and pituitary of the seven year's old (sex reversal stage) E. coioides, it may be involved in sex reverse.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Oceanography
研究领域[WOS]	Oceanography
关键词[WOS]	COMPLETE GENOME SEQUENCE ; WARFARIN SENSITIVITY ; TISSUE DISTRIBUTION ; IDENTIFICATION ; HORMONE
收录类别	SCI
资助信息	CAS-TWAS (Chinese Academy of Sciences (Beijing and Wuhan Branch) - Third World Academy of Sciences (Italy))
语种	英语
WOS记录号	WOS:000329600100006
公开日期	2014-08-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19974]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Kanyakumari 629502, Tamil Nadu, India 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Citarasu, T.,Michaelbabu, M.,Zhou, L.,et al. cDNA cloning and expression of vitamin-K Epoxide Reductase (VKOR) gene from orange spotted grouper Epinephelus coioides[J]. INDIAN JOURNAL OF GEO-MARINE SCIENCES,2013,42(5):573-579.
APA	Citarasu, T.,Michaelbabu, M.,Zhou, L.,Yang, W.,&Gui, J. F..(2013).cDNA cloning and expression of vitamin-K Epoxide Reductase (VKOR) gene from orange spotted grouper Epinephelus coioides.INDIAN JOURNAL OF GEO-MARINE SCIENCES,42(5),573-579.
MLA	Citarasu, T.,et al."cDNA cloning and expression of vitamin-K Epoxide Reductase (VKOR) gene from orange spotted grouper Epinephelus coioides".INDIAN JOURNAL OF GEO-MARINE SCIENCES 42.5(2013):573-579.