Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish

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	Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish
	Dan, Cheng 1; Mei, Jie 2; Wang, Da 1; Gui, Jian-Fang 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
	2013
卷号	9 期号:10 页码:1043-1049
关键词	Yellow catfish Sex-specific marker genome walking YY super-males All-male fish
ISSN号	1449-2288
通讯作者	Mei, J (reprint author), Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China.
中文摘要	Pf62-Y and Pf62-X is a pair of allelic Y chromosome-linked and X chromosome-linked markers, and have been used to identify YY super-males, XY males and XX females for commercial production of all-male populations in yellow catfish (Pelteobagrus fulvidraco). However, the SCAR primers used previously have only two nucleotide difference, which restricts the wide utility because of nucleotide polymorphism. In this study, a continuous 8102 bp Pf62-Y sequence and a 5362 bp Pf62-X sequence have been cloned by genome walking, and significant genetic differentiation has been revealed between the corresponding X and Y chromosome allele sequences. Moreover, three pairs of primers were designed to efficiently identify YY super-males, XY males and XX females in an artificial breeding population, and to distinguish XY males and XX females in various wild populations. Together, the three new sex-specific genetic markers develop a highly stable and efficient method for genetic sex identification and sex control application in sustainable aquaculture of all-male yellow catfish.
英文摘要	Pf62-Y and Pf62-X is a pair of allelic Y chromosome-linked and X chromosome-linked markers, and have been used to identify YY super-males, XY males and XX females for commercial production of all-male populations in yellow catfish (Pelteobagrus fulvidraco). However, the SCAR primers used previously have only two nucleotide difference, which restricts the wide utility because of nucleotide polymorphism. In this study, a continuous 8102 bp Pf62-Y sequence and a 5362 bp Pf62-X sequence have been cloned by genome walking, and significant genetic differentiation has been revealed between the corresponding X and Y chromosome allele sequences. Moreover, three pairs of primers were designed to efficiently identify YY super-males, XY males and XX females in an artificial breeding population, and to distinguish XY males and XX females in various wild populations. Together, the three new sex-specific genetic markers develop a highly stable and efficient method for genetic sex identification and sex control application in sustainable aquaculture of all-male yellow catfish.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
关键词[WOS]	SOLE CYNOGLOSSUS-SEMILAEVIS ; PELTEOBAGRUS-FULVIDRACO RICHARDSON ; CARASSIUS-AURATUS GIBELIO ; AFLP-BASED APPROACH ; CYPRINUS-CARPIO ; CLONAL DIVERSITY ; DNA FRAGMENTS ; COMMON CARP ; LINKAGE MAP ; IDENTIFICATION
收录类别	SCI
资助信息	special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [2009030406]; National Key Basic Research Program [2010CB126301]; Innovation Project of Chinese Academy of Sciences [KSCX2-EW-N-04]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]
语种	英语
WOS记录号	WOS:000330121700004
公开日期	2014-08-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19972]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Dan, Cheng,Mei, Jie,Wang, Da,et al. Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2013,9(10):1043-1049.
APA	Dan, Cheng,Mei, Jie,Wang, Da,&Gui, Jian-Fang.(2013).Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,9(10),1043-1049.
MLA	Dan, Cheng,et al."Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 9.10(2013):1043-1049.