Generation of a monkey with MECP2 mutations by TALEN-based gene targeting

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	Generation of a monkey with MECP2 mutations by TALEN-based gene targeting
	Liu, Z ; Zhou, X ; Zhu, Y ; Chen, ZF ; Yu, B ; Wang, Y ; Zhang, CC ; Nie, YH ; Sang, X ; Cai, YJ ; Zhang, YF ; Zhang, C ; Zhou, WH ; Sun, Q ; Qiu, ZL
刊名	NEUROSCIENCE BULLETIN
	2014
卷号	30 期号:3 页码:381-386
关键词	CPG-BINDING PROTEIN-2 ONE-STEP GENERATION CYNOMOLGUS MONKEY RETT-SYNDROME MICE CHROMATIN MOUSE
ISSN号	1673-7067
通讯作者	Qiu, ZL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China.,qsun@ion.ac.cn ; zqiu@ion.ac.cn
英文摘要	Gene editing in model organisms has provided critical insights into brain development and diseases. Here, we report the generation of a cynomolgus monkey (Macaca fascicularis) carrying MECP2 mutations using transcription activator-like effector nucleases (TALENs)-mediated gene targeting. After injecting TALENs mRNA into monkey zygotes achieved by in vitro fertilization and embryo transplantation into surrogate monkeys, we obtained one male newborn monkey with an MECP2 deletion caused by frameshifting mutation in various tissues. The monkey carrying the MECP2 mutation failed to survive after birth, due to either the toxicity of TALENs or the critical requirement of MECP2 for neural development. The level of MeCP2 protein was essentially depleted in the monkey's brain. This study demonstrates the feasibility of introducing genetic mutations in non-human primates by site-specific gene-editing methods.
学科主题	Neurosciences
收录类别	SCI
资助信息	National Basic Research Development Program of China [2011CBA00400, 2011CB809102]; CAS Strategic Priority Research Program of China [XDB02050400]; National Key Technology R&D Program of China [2014BAI03B00]; CAS Hundreds of Talents Program of China; National Science Foundation of China [91232712]
语种	英语
公开日期	2014-07-30
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2604]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Liu, Z,Zhou, X,Zhu, Y,et al. Generation of a monkey with MECP2 mutations by TALEN-based gene targeting[J]. NEUROSCIENCE BULLETIN,2014,30(3):381-386.
APA	Liu, Z.,Zhou, X.,Zhu, Y.,Chen, ZF.,Yu, B.,...&Qiu, ZL.(2014).Generation of a monkey with MECP2 mutations by TALEN-based gene targeting.NEUROSCIENCE BULLETIN,30(3),381-386.
MLA	Liu, Z,et al."Generation of a monkey with MECP2 mutations by TALEN-based gene targeting".NEUROSCIENCE BULLETIN 30.3(2014):381-386.