Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection
	Zhang, Wenjing 1,2; Tao, Junyan 1,2; Yang, Xiaoping 1,2; Yang, Zhuliang3 ; Zhang, Li 4; Liu, Hongsheng 4; Wu, Kailang 1,2; Wu, Jianguo 1,2
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2014-07-04
卷号	449 期号:3 页码:307-312
关键词	Enterovirus 71 (EV71) Virus infection Hand-foot-mouth disease (HFMD) Ganoderma lucidum triterpenoids (GLTs) Antiviral activity Potential therapeutic antiviral agents
ISSN号	0006-291X
通讯作者	Wu,JG (reprint author),Wuhan Univ,State Key Lab Virol,Wuhan 430072,Peoples R China. ; jwu@whu.edu.cn
英文摘要	Enterovirus 71 (EV71) is a major causative agent for hand, foot and mouth disease (HFMD), and fatal neurological and systemic complications in children. However, there is currently no clinical approved antiviral drug available for the prevention and treatment of the viral infection. Here, we evaluated the antiviral activities of two Ganoderma lucidum triterpenoids (GLTs), Lanosta-7,9(11),24-trien-3-one,15;26-dihydroxy (GLTA) and Ganoderic acid Y (GLTB), against EV71 infection. The results showed that the two natural compounds display significant anti-EV71 activities without cytotoxicity in human rhabdomyosarcoma (RD) cells as evaluated by 3-(4,5-Dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay. The mechanisms by which the two compounds affect EV71 infection were further elucidated by three action modes using Ribavirin, a common antiviral drug, as a positive control. The results suggested that GLTA and GLTB prevent EV71 infection through interacting with the viral particle to block the adsorption of virus to the cells. In addition, the interactions between EV71 virion and the compounds were predicated by computer molecular docking, which illustrated that GLTA and GLTB may bind to the viral capsid protein at a hydrophobic pocket (F site), and thus may block uncoating of EV71. Moreover, we demonstrated that GLTA and GLTB significantly inhibit the replication of the viral RNA (vRNA) of EV71 replication through blocking EV71 uncoating. Thus, GLTA and GLTB may represent two potential therapeutic agents to control and treat EV71 infection. (C) 2014 Elsevier Inc. All rights reserved.
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	CHINESE HERBAL MEDICINE ; MOUTH-DISEASE ; IN-VITRO ; ANTICANCER ; FOOT ; HAND
收录类别	SCI
资助信息	Major State Basic Research Development Program (973 Program) [2012CB518900];National Natural Science Foundation of China [31230005, 81171525];National Mega Project on Major Infectious Disease Prevention [2012ZX10002006-003, 2012ZX10004-207];National Mega Project on Major Drug Development [2011ZX09401-302]
语种	英语
WOS记录号	WOS:000337781500009
公开日期	2014-07-28
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/18209]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Wuhan Univ, State Key Lab Virol, Wuhan 430072, Peoples R China 2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China 4.Liaoning Univ, Dept Acad Sci, Shenyang 110036, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wenjing,Tao, Junyan,Yang, Xiaoping,et al. Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,449(3):307-312.
APA	Zhang, Wenjing.,Tao, Junyan.,Yang, Xiaoping.,Yang, Zhuliang.,Zhang, Li.,...&Wu, Jianguo.(2014).Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,449(3),307-312.
MLA	Zhang, Wenjing,et al."Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 449.3(2014):307-312.