Structural analysis of heteropolysaccharide from Saccharina japonica and its derived oligosaccharides

	Structural analysis of heteropolysaccharide from Saccharina japonica and its derived oligosaccharides
	Jin, Weihua 1,2; Zhang, Wenjing1,2 ; Wang, Jing1 ; Ren, Sumei 3; Song, Ni 3; Zhang, Quanbin 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2013-11-01
卷号	62 页码:697-704
关键词	ESI-MS Oligosaccharides Saccharina japonica
ISSN号	0141-8130
通讯作者	Zhang, QB
中文摘要	Degraded fucoidan (F1) was desulfated by DMSO-MeOH. And anion exchange chromatography was performed to fractionate desulfated F1 (ds-F1) into five fractions. Electrospray ionization mass spectrometry (ESI-MS) showed that each fraction contained at least one set of neutral and/or sulfated fucooligosaccharides in the form of methyl glycosides. And the structures of oligomeric fragments were characterized by ESI-CID-MS/MS and ESI-CID-MS/MS/MS. In addition, more structural features were shown by NMR. Therefore, it was concluded that LF1 contained a backbone of (1 -> 3)-linked fucopyranose residues sulfated at C-4 and branched at C-2 by fucopyranose residues and fucoglucuronomannan, fucoglucuronan, galactan and xylan were found in LF-5. Finally, it was concluded that F1 was the middle component, which contained the information of both F0.5 and F2, indicating that the differences between F1 and F0.5, F2 might be derived primarily from the different needs of algae itself. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要	Degraded fucoidan (F1) was desulfated by DMSO-MeOH. And anion exchange chromatography was performed to fractionate desulfated F1 (ds-F1) into five fractions. Electrospray ionization mass spectrometry (ESI-MS) showed that each fraction contained at least one set of neutral and/or sulfated fucooligosaccharides in the form of methyl glycosides. And the structures of oligomeric fragments were characterized by ESI-CID-MS/MS and ESI-CID-MS/MS/MS. In addition, more structural features were shown by NMR. Therefore, it was concluded that LF1 contained a backbone of (1 -> 3)-linked fucopyranose residues sulfated at C-4 and branched at C-2 by fucopyranose residues and fucoglucuronomannan, fucoglucuronan, galactan and xylan were found in LF-5. Finally, it was concluded that F1 was the middle component, which contained the information of both F0.5 and F2, indicating that the differences between F1 and F0.5, F2 might be derived primarily from the different needs of algae itself. (C) 2013 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	TANDEM MASS-SPECTROMETRY ; SULFATED POLYSACCHARIDES ; BROWN SEAWEEDS ; ANTICOAGULANT ACTIVITY ; CID-MS/MS ; FUCOIDAN ; DISACCHARIDES ; FUCANS ; GALACTOFUCAN ; DISSOCIATION
收录类别	SCI
原文出处	10.1016/j.ijbiomac.2013.10.017
语种	英语
WOS记录号	WOS:000329881200103
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16720]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ocean Univ China, Coll Med & Pharmaceut, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Jin, Weihua,Zhang, Wenjing,Wang, Jing,et al. Structural analysis of heteropolysaccharide from Saccharina japonica and its derived oligosaccharides[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2013,62:697-704.
APA	Jin, Weihua,Zhang, Wenjing,Wang, Jing,Ren, Sumei,Song, Ni,&Zhang, Quanbin.(2013).Structural analysis of heteropolysaccharide from Saccharina japonica and its derived oligosaccharides.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,62,697-704.
MLA	Jin, Weihua,et al."Structural analysis of heteropolysaccharide from Saccharina japonica and its derived oligosaccharides".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 62(2013):697-704.