Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas

	Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas
	Guevelou, Eric 1; Huvet, Arnaud 1; Sussarellu, Rossana 1,2; Milan, Massimo 3; Guo, Ximing 4; Li, Li 5; Zhang, Guofan 5; Quillien, Virgile 1; Daniel, Jean-Yves 1; Quere, Claudie 1
刊名	JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY
	2013-07-01
卷号	183 期号:5 页码:597-611
关键词	Marine bivalve Crassostrea gigas Hypoxia AMP-activated protein kinase Alternative splicing Adductor muscle
ISSN号	0174-1578
通讯作者	Corporeau, C
中文摘要	AMP-activated protein kinase alpha (AMPK alpha) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase alpha (AMPK alpha) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPK alpha. In the smooth muscle, a truncated isoform of AMPK alpha was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism was maintained over the 6 h of hypoxia, as mitochondrial citrate synthase activity remained constant. In contrast, in striated muscle, hypoxia did not induce any significant modification of neither truncated AMPK alpha nor AKT protein content, and citrate synthase activity was altered after 6 h of hypoxia. Together, our results demonstrate that hypoxia response is specific to muscle type in Pacific oyster, and that truncated AMPK alpha and AKT proteins might be involved in maintaining aerobic metabolism in smooth muscle. Such regulation might occur in vivo during tidal intervals that cause up to 6 h of hypoxia.
英文摘要	AMP-activated protein kinase alpha (AMPK alpha) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase alpha (AMPK alpha) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPK alpha. In the smooth muscle, a truncated isoform of AMPK alpha was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism was maintained over the 6 h of hypoxia, as mitochondrial citrate synthase activity remained constant. In contrast, in striated muscle, hypoxia did not induce any significant modification of neither truncated AMPK alpha nor AKT protein content, and citrate synthase activity was altered after 6 h of hypoxia. Together, our results demonstrate that hypoxia response is specific to muscle type in Pacific oyster, and that truncated AMPK alpha and AKT proteins might be involved in maintaining aerobic metabolism in smooth muscle. Such regulation might occur in vivo during tidal intervals that cause up to 6 h of hypoxia.
学科主题	Physiology ; Zoology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Physiology ; Zoology
研究领域[WOS]	Physiology ; Zoology
关键词[WOS]	METABOLIC-RATE DEPRESSION ; RAT SKELETAL-MUSCLE ; GLUCOSE-TRANSPORT ; REPRODUCTIVE-CYCLE ; MOLECULAR-CLONING ; OOCYTE MATURATION ; CELLULAR-ENERGY ; GENE-EXPRESSION ; SUBUNIT GENE ; AKT ACTIVITY
收录类别	SCI
原文出处	10.1007/s00360-013-0743-6
语种	英语
WOS记录号	WOS:000320733500002
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16717]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.IFREMER, Ctr Bretagne ZI Pointe Diable, LEMAR, UMR 6539, F-29280 Plouzane, France 2.CNRS, IUEM UBO, UMR 6539, LEMAR, F-29280 Plouzane, France 3.Univ Padua, Dept Comparat Biomed & Food Sci, Legnaro, Italy 4.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 5.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Guevelou, Eric,Huvet, Arnaud,Sussarellu, Rossana,et al. Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas[J]. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,2013,183(5):597-611.
APA	Guevelou, Eric.,Huvet, Arnaud.,Sussarellu, Rossana.,Milan, Massimo.,Guo, Ximing.,...&Corporeau, Charlotte.(2013).Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas.JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,183(5),597-611.
MLA	Guevelou, Eric,et al."Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas".JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY 183.5(2013):597-611.