Molecular cloning and expression-profile analysis of sea cucumber DNA (Cytosine-5)-methyltransferase 1 and methyl-CpG binding domain type 2/3 genes during aestivation

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Molecular cloning and expression-profile analysis of sea cucumber DNA (Cytosine-5)-methyltransferase 1 and methyl-CpG binding domain type 2/3 genes during aestivation
	Zhao, Ye1,2 ; Chen, Muyan 3; Su, Lin 1,2; Wang, Tianming 4; Liu, Shilin 1; Yang, Hongsheng1
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
	2013-05-01
卷号	165 期号:1 页码:26-35
关键词	Apostichopus japonicus Selenka Aestivation dnmt1 mbd2/3 DNA methylation
ISSN号	1096-4959
通讯作者	Chen, MY
中文摘要	The sea cucumber Apostichopus japonicus Selenka survives high summer temperature by entering aestivation, characterized by hypometabolism and global gene silencing. We investigated the hypothesis that aestivation is associated with DNA methylation-dependent epigenetic mechanisms by cloning, sequencing and measuring the transcript abundances of two genes dnmt1 and mbd2/3, which comprise the DNA methylation system in A. japonicus Selenka. The deduced amino acid sequences and characteristic motifs of sea cucumber DNMT1 and MBD2/3 showed high homology to those of their mammalian counterparts. Quantitative real-time RT-PCR analysis showed that dnmt1 and mbd2/3 genes were similarly expressed in all four tissues examined (intestine, respiratory tree, muscle and body wall). dnmt1 expression in the intestine was up-regulated during deep aestivation (P < 0.05), while mbd2/3 was over-expressed in both the intestine and respiratory tree during the same period (P < 0.01). No differences in expression levels were observed between other tissues. The results of this study suggest that DNA methylation may be involved in transcriptional silencing, and that the intestine is the major site for epigenetic regulation during aestivation in the sea cucumber. (C) 2013 Elsevier Inc. All rights reserved.
英文摘要	The sea cucumber Apostichopus japonicus Selenka survives high summer temperature by entering aestivation, characterized by hypometabolism and global gene silencing. We investigated the hypothesis that aestivation is associated with DNA methylation-dependent epigenetic mechanisms by cloning, sequencing and measuring the transcript abundances of two genes dnmt1 and mbd2/3, which comprise the DNA methylation system in A. japonicus Selenka. The deduced amino acid sequences and characteristic motifs of sea cucumber DNMT1 and MBD2/3 showed high homology to those of their mammalian counterparts. Quantitative real-time RT-PCR analysis showed that dnmt1 and mbd2/3 genes were similarly expressed in all four tissues examined (intestine, respiratory tree, muscle and body wall). dnmt1 expression in the intestine was up-regulated during deep aestivation (P < 0.05), while mbd2/3 was over-expressed in both the intestine and respiratory tree during the same period (P < 0.01). No differences in expression levels were observed between other tissues. The results of this study suggest that DNA methylation may be involved in transcriptional silencing, and that the intestine is the major site for epigenetic regulation during aestivation in the sea cucumber. (C) 2013 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Zoology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Zoology
研究领域[WOS]	Biochemistry & Molecular Biology ; Zoology
关键词[WOS]	APOSTICHOPUS-JAPONICUS SELENKA ; PROTEINS ; TRANSCRIPTION ; ANIMALS ; GENOME ; REPRESSION ; DEPRESSION ; INTESTINE ; CHROMATIN ; PATTERNS
收录类别	SCI
原文出处	10.1016/j.cbpb.2013.02.009
语种	英语
WOS记录号	WOS:000318464900004
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16482]
专题	海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China 4.Zhejiang Univ, Ocean Res Ctr Zhoushan, Zhoushan, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Ye,Chen, Muyan,Su, Lin,et al. Molecular cloning and expression-profile analysis of sea cucumber DNA (Cytosine-5)-methyltransferase 1 and methyl-CpG binding domain type 2/3 genes during aestivation[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2013,165(1):26-35.
APA	Zhao, Ye,Chen, Muyan,Su, Lin,Wang, Tianming,Liu, Shilin,&Yang, Hongsheng.(2013).Molecular cloning and expression-profile analysis of sea cucumber DNA (Cytosine-5)-methyltransferase 1 and methyl-CpG binding domain type 2/3 genes during aestivation.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,165(1),26-35.
MLA	Zhao, Ye,et al."Molecular cloning and expression-profile analysis of sea cucumber DNA (Cytosine-5)-methyltransferase 1 and methyl-CpG binding domain type 2/3 genes during aestivation".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 165.1(2013):26-35.