The major fimbrial subunit protein of Edwardsiella tarda: Vaccine potential, adjuvant effect, and involvement in host infection

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	The major fimbrial subunit protein of Edwardsiella tarda: Vaccine potential, adjuvant effect, and involvement in host infection
	Wang, Chong 1,2; Hu, Yong-hua 1; Chi, Heng 1; Sun, Li 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013-09-01
卷号	35 期号:3 页码:858-865
关键词	Edwardsiella tarda Fimbrial Virulence Vaccine Adjuvant
ISSN号	1050-4648
通讯作者	Sun, L
中文摘要	Edwardsiella tarda is a Gram-negative bacterium that is reckoned one of the most severe fish pathogens. In this study, we analyzed the biological properties of the E. tarda major fimbrial subunit protein, FimA. We found that mutation of fimA resulted in defective biofilm growth, attenuated infectivity against host cells, and impaired ability to disseminate into and colonize host tissues following experimental infection. When used as a subunit vaccine, recombinant FimA (rFimA) elicited a high level of protection in turbot (Scophthalmus maximus) against lethal E. tarda challenge. Immunological analysis showed that rFimA vaccination induced production of specific serum antibodies that bound to live E. tarda via interaction with the FimA on bacterial cells, and that antibody-E. tarda interaction blocked bacterial infection. Furthermore, passive immunization of turbot with anti-rFimA serum before E. tarda infection reduced bacterial loads in fish tissues to significant extents. To examine the adjuvant potential of FimA, turbot were vaccinated with rVhhP2, a protective Vibrio harveyi antigen, in the presence or absence of rFimA. Subsequent analysis showed that the presence of rFimA significantly augmented the protectivity of rVhhP2. ELISA and quantitative real time RT-PCR showed that rFimA significantly increased rVhhP2-specific serum antibody production and enhanced the expression of immune relevant genes. Taken together, these results indicate that FimA is a virulence-associated protein that possesses vaccine as well as adjuvant potentials, and that the immunoprotectivity of FimA is most likely due to its ability to induce specific immune response that inhibits E. tarda infection. (C) 2013 Elsevier Ltd. All rights reserved.
英文摘要	Edwardsiella tarda is a Gram-negative bacterium that is reckoned one of the most severe fish pathogens. In this study, we analyzed the biological properties of the E. tarda major fimbrial subunit protein, FimA. We found that mutation of fimA resulted in defective biofilm growth, attenuated infectivity against host cells, and impaired ability to disseminate into and colonize host tissues following experimental infection. When used as a subunit vaccine, recombinant FimA (rFimA) elicited a high level of protection in turbot (Scophthalmus maximus) against lethal E. tarda challenge. Immunological analysis showed that rFimA vaccination induced production of specific serum antibodies that bound to live E. tarda via interaction with the FimA on bacterial cells, and that antibody-E. tarda interaction blocked bacterial infection. Furthermore, passive immunization of turbot with anti-rFimA serum before E. tarda infection reduced bacterial loads in fish tissues to significant extents. To examine the adjuvant potential of FimA, turbot were vaccinated with rVhhP2, a protective Vibrio harveyi antigen, in the presence or absence of rFimA. Subsequent analysis showed that the presence of rFimA significantly augmented the protectivity of rVhhP2. ELISA and quantitative real time RT-PCR showed that rFimA significantly increased rVhhP2-specific serum antibody production and enhanced the expression of immune relevant genes. Taken together, these results indicate that FimA is a virulence-associated protein that possesses vaccine as well as adjuvant potentials, and that the immunoprotectivity of FimA is most likely due to its ability to induce specific immune response that inhibits E. tarda infection. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	OUTER-MEMBRANE PROTEIN ; PROTECTIVE IMMUNOGEN ; BIOFILM FORMATION ; ESCHERICHIA-COLI ; PSEUDOMONAS-AERUGINOSA ; SCOPHTHALMUS-MAXIMUS ; VIBRIO-ANGUILLARUM ; TYPE-1 FIMBRIAE ; URINARY-TRACT ; IDENTIFICATION
收录类别	SCI
原文出处	10.1016/j.fsi.2013.06.021
语种	英语
WOS记录号	WOS:000324511700028
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16651]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chong,Hu, Yong-hua,Chi, Heng,et al. The major fimbrial subunit protein of Edwardsiella tarda: Vaccine potential, adjuvant effect, and involvement in host infection[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):858-865.
APA	Wang, Chong,Hu, Yong-hua,Chi, Heng,&Sun, Li.(2013).The major fimbrial subunit protein of Edwardsiella tarda: Vaccine potential, adjuvant effect, and involvement in host infection.FISH & SHELLFISH IMMUNOLOGY,35(3),858-865.
MLA	Wang, Chong,et al."The major fimbrial subunit protein of Edwardsiella tarda: Vaccine potential, adjuvant effect, and involvement in host infection".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):858-865.