中华仙影海葵溶细胞毒素的初步分离及稳定性研究

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	中华仙影海葵溶细胞毒素的初步分离及稳定性研究
	史文军 ; 张朝晖 ; 秦松 ; 吴建平
刊名	中国海洋药物
	2013
卷号	32 期号:5 页码:14-20
关键词	中华仙影海葵溶细胞活性活性组分稳定性
ISSN号	1002-3461
其他题名	Preliminary isolation and stability analysis of cytolytic toxins from Cereus sinensis Verrill
通讯作者	张朝晖 E-mail:zzh6495@eyou.com
产权排序	江苏大学药学院;江苏省海洋水产研究所;中科院烟台海岸带研究所;
中文摘要	目的初步分离中华仙影海葵溶细胞毒素(Cereus sinensis Verrill cytolytic toxins,CCT)并探讨其体外溶细胞活性及影响因素。方法采用冻融、匀浆、水浸和离心的方法获取海葵粗毒,通过硫酸铵盐析和Sephadex G-100凝胶色谱法将海葵粗毒60%~90%盐析段分为5个组分,检测5个组分溶细胞活性并收集活性较强的第1组分(CCT);测定其半数溶细胞分数(CU50)、最适孵育温度及最适pH,探讨EDTA和金属离子对溶细胞活性的影响。结果经硫酸铵盐析和分子筛处理后,中华仙影海葵溶细胞活性成分主要集中在第1组分(CCT),CCT溶细胞活性的CU50为7.76μg·mL...
英文摘要	Objective To preliminarily isolate cytolytic toxins from Cereus sinensis Verrill and investigate the factors influencing their cytolytic activity. Methods Crude venom from sea anemone was successively obtained by freeze-thaw, homogenate,water extraction and centrifugation. Five parts were isolated from the 60%-90% fraction by ammonium su/fate precipitation and Sephadex G-100 size-exclusion chroma- tograph. The first section （CCT） was shown to be the most potential one by measuring cytolytic activi- ty of them; CU50 ,the best incubation temperature and the best pH of CCT were detected, then the in- fluence of EDTA and metal cations to CCT were investigated. Result CCT was confirmed to be the most potential part by ammonium sulfate precipitation and Sephadex G-100 size-exclusion chromatograph. The CU50 of CCT was 7. 76μg mL-1. the best incubation temperature of CU50 was 40℃ and the best pH of CCT was 8; in vitro cytolytic study of CCT showed that the its cytolytic activity was influenced by EDTA and metal cations mostly. Conclusion The active fraction from Cereus sinensis Verrill was pre- liminarily isolated by ammonium sulfate precipitation and Sephadex G-100 size-exclusion chromato- graph, and the factors influencing cytolytic activity of CCT were detected. This study provides support to discover purified compound subsequently
学科主题	天然药物学
收录类别	CSCD
资助信息	2012年南通市生物技术和新医药计划项目(AS20120006)资助
语种	中文
CSCD记录号	CSCD:5030601
公开日期	2014-07-07
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6882]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
推荐引用方式 GB/T 7714	史文军,张朝晖,秦松,等. 中华仙影海葵溶细胞毒素的初步分离及稳定性研究[J]. 中国海洋药物,2013,32(5):14-20.
APA	史文军,张朝晖,秦松,&吴建平.(2013).中华仙影海葵溶细胞毒素的初步分离及稳定性研究.中国海洋药物,32(5),14-20.
MLA	史文军,et al."中华仙影海葵溶细胞毒素的初步分离及稳定性研究".中国海洋药物 32.5(2013):14-20.