Graphene Oxide Wrapped SERS Tags: Multifunctional Platforms toward Optical Labeling, Photothermal Ablation of Bacteria, and the Monitoring of Killing Effect

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Graphene Oxide Wrapped SERS Tags: Multifunctional Platforms toward Optical Labeling, Photothermal Ablation of Bacteria, and the Monitoring of Killing Effect
	Lin, Donghai 1; Qin, Tianqi 1; Wang, Yunqing2 ; Sun, Xiuyan 1; Chen, Lingxin2
刊名	ACS APPLIED MATERIALS & INTERFACES
	2014-01-22
卷号	6 期号:2 页码:1320-1329
关键词	graphene oxide gold nanoparticles SERS tags bacteria photothermal ablation
ISSN号	1944-8244
通讯作者	Wang, YQ (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. yqwang@yic.ac.cn ; lxchen@yic.ac.cn
产权排序	[Lin, Donghai; Qin, Tianqi; Sun, Xiuyan] Yantai Univ, Sch Pharm, Yantai 264005, Peoples R China; [Wang, Yunqing; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
中文摘要	As novel optical nanoprobes, surface-enhanced Raman scattering (SERS) tags have drawn growing interests in the application of biomedical imaging and phototherapies. Herein, we demonstrated a novel in situ synthesis strategy for GO wrapped gold nanocluster SERS tags by using a tris(2,2'-bipyridyl)ruthenium-(II) chloride (Rubpy)/GO nanohybrid as a complex Raman reporter, inspired by the role of GO as an artificial receptor for various dyes. The introduction of GO in the synthesis procedure provided systematic solutions for controlling several key parameters of SERS tags, including reproducibility, sensitivity, and colloidal and signal stability. An additional interesting thermal-sensitive SERS property (SERS intensity decreased upon increasing the temperature) was also achieved due to the heat-induced release/redistribution of reporter molecules adsorbed on GO. Combining the synergic effect of these features, we further fabricated multifunctional, aldehyde group conjugated Au@Rubpy/GO SEAS tags for optical labeling and photothermal ablation of bacteria. Sensitive Raman imaging of gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria could be realized, and satisfactory photothermal killing efficacy for both bacteria was achieved. Our results also demonstrated the correlation among the SEAS intensity decrease ratio, bacteria survival rate, and the terminal temperature of the tag-bacteria suspension, showing the possibility to use SERS assay to measure antibacterial response during the photothermal process using this tag.
英文摘要	As novel optical nanoprobes, surface-enhanced Raman scattering (SERS) tags have drawn growing interests in the application of biomedical imaging and phototherapies. Herein, we demonstrated a novel in situ synthesis strategy for GO wrapped gold nanocluster SERS tags by using a tris(2,2'-bipyridyl)ruthenium-(II) chloride (Rubpy)/GO nanohybrid as a complex Raman reporter, inspired by the role of GO as an artificial receptor for various dyes. The introduction of GO in the synthesis procedure provided systematic solutions for controlling several key parameters of SERS tags, including reproducibility, sensitivity, and colloidal and signal stability. An additional interesting thermal-sensitive SERS property (SERS intensity decreased upon increasing the temperature) was also achieved due to the heat-induced release/redistribution of reporter molecules adsorbed on GO. Combining the synergic effect of these features, we further fabricated multifunctional, aldehyde group conjugated Au@Rubpy/GO SEAS tags for optical labeling and photothermal ablation of bacteria. Sensitive Raman imaging of gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria could be realized, and satisfactory photothermal killing efficacy for both bacteria was achieved. Our results also demonstrated the correlation among the SEAS intensity decrease ratio, bacteria survival rate, and the terminal temperature of the tag-bacteria suspension, showing the possibility to use SERS assay to measure antibacterial response during the photothermal process using this tag.
学科主题	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]	Science & Technology - Other Topics ; Materials Science
关键词[WOS]	ENHANCED RAMAN-SCATTERING ; FUNCTIONALIZED SILVER NANOPARTICLES ; SHELL NANOPARTICLES ; GOLD NANOPARTICLES ; DRUG-DELIVERY ; SURFACE ; SPECTROSCOPY ; THERAPY ; ANTIAGGREGATION ; FABRICATION
收录类别	SCI
资助信息	National Natural Science Foundation of China [81102415, 21275158]; Natural Science Foundation of Shandong Province of China [ZR2010BQ012]; Science and Technology Development Plan of Yantai [2011071]; One Hundred Person Project of the Chinese Academy of Sciences
原文出处	http://dx.doi.org/10.1021/am405396k
语种	英语
WOS记录号	WOS:000330201900080
公开日期	2014-07-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6909]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Yantai Univ, Sch Pharm, Yantai 264005, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Lin, Donghai,Qin, Tianqi,Wang, Yunqing,et al. Graphene Oxide Wrapped SERS Tags: Multifunctional Platforms toward Optical Labeling, Photothermal Ablation of Bacteria, and the Monitoring of Killing Effect[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(2):1320-1329.
APA	Lin, Donghai,Qin, Tianqi,Wang, Yunqing,Sun, Xiuyan,&Chen, Lingxin.(2014).Graphene Oxide Wrapped SERS Tags: Multifunctional Platforms toward Optical Labeling, Photothermal Ablation of Bacteria, and the Monitoring of Killing Effect.ACS APPLIED MATERIALS & INTERFACES,6(2),1320-1329.
MLA	Lin, Donghai,et al."Graphene Oxide Wrapped SERS Tags: Multifunctional Platforms toward Optical Labeling, Photothermal Ablation of Bacteria, and the Monitoring of Killing Effect".ACS APPLIED MATERIALS & INTERFACES 6.2(2014):1320-1329.