A promoter analysis of MOTHER OF FT AND TFL1 1 (JcMFT1), a seed-preferential gene from the biofuel plant Jatropha curcas
Zeng-Fu Xu; Zeng-Fu Xu; Zeng-Fu Xu; Zeng-Fu Xu; Zeng-Fu Xu; Zeng-Fu Xu; Zeng-Fu Xu; Zeng-Fu Xu; Zeng-Fu Xu; Zeng-Fu Xu
刊名Journal of Plant Research
2014
卷号127期号:4页码:513-524
中文摘要

MOTHER OF FT AND TFL1 (MFT)-like genes belong to the phosphatidylethanoamine-binding protein (PEBP) gene family in plants. In contrast to their homologs FLOWERING LOCUS T (FT)-like and TERMINAL FLOWER 1 (TFL1)-like genes, which are involved in the regulation of the flowering time pathway, MFT-like genes function mainly during seed development and germination. In this study, a full-length cDNA of the MFT-like gene JcMFT1 from the biodiesel plant Jatropha curcas (L.) was isolated and found to be highly expressed in seeds. The promoter of JcMFT1 was cloned and characterized in transgenic Arabidopsis. A histochemical β-glucuronidase (GUS) assay indicated that the JcMFT1 promoter was predominantly expressed in both embryos and endosperms of transgenic Arabidopsis seeds. Fluorometric GUS analysis revealed that the JcMFT1 promoter was highly active at the mid to late stages of seed development. After seed germination, the JcMFT1 promoter activity decreased gradually. In addition, both the JcMFT1 expression in germinating Jatropha embryos and its promoter activity in germinating Arabidopsis embryos were induced by abscisic acid (ABA), possibly due to two ABA-responsive elements, a G-box and an RY repeat, in the JcMFT1 promoter region. These results show that the JcMFT1 promoter is seed-preferential and can be used to control transgene expression in the seeds of Jatropha and other transgenic plants.

公开日期2014-07-02
内容类型期刊论文
源URL[http://ir.xtbg.org.cn/handle/353005/5002]  
专题西双版纳热带植物园_能源植物分子育种研究组
推荐引用方式
GB/T 7714
Zeng-Fu Xu,Zeng-Fu Xu,Zeng-Fu Xu,et al. A promoter analysis of MOTHER OF FT AND TFL1 1 (JcMFT1), a seed-preferential gene from the biofuel plant Jatropha curcas[J]. Journal of Plant Research,2014,127(4):513-524.
APA Zeng-Fu Xu.,Zeng-Fu Xu.,Zeng-Fu Xu.,Zeng-Fu Xu.,Zeng-Fu Xu.,...&Zeng-Fu Xu.(2014).A promoter analysis of MOTHER OF FT AND TFL1 1 (JcMFT1), a seed-preferential gene from the biofuel plant Jatropha curcas.Journal of Plant Research,127(4),513-524.
MLA Zeng-Fu Xu,et al."A promoter analysis of MOTHER OF FT AND TFL1 1 (JcMFT1), a seed-preferential gene from the biofuel plant Jatropha curcas".Journal of Plant Research 127.4(2014):513-524.
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