Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers
	Zhao, Bin ; Yan, Juan ; Wang, Dongfang ; Ge, Zhilei ; He, Shijiang ; He, Dannong ; Song, Shiping（宋世平） ; Fan, Chunhai（樊春海）
刊名	SMALL
	2013
卷号	9 期号:15 页码:CONCATENATE(Sheet1!I47,-Sheet1!J47)
ISSN号	1613-6810; 1613-6829
英文摘要	There are still challenges for the development of multifunctional carbon nanotubes (CNTs). Here, a multiwalled carbon nanotube (MWCNT)-based rolling circle amplification system (CRCAS) is reported which allows in situ rolling circle replication of DNA primer on the surface of MWCNTs to create a long single-strand DNA (ssDNA) where a large number of nanoparticles or proteins could be loaded, forming a nano-biohybridized 3D structure with a powerful signal amplification ability. In this strategy, the binding ability of proteins, hybridization, replication ability of DNA, and the catalytical ability of enzymes are integrated on a single carbon nanotube. The CRCAS is then used to develop colorimetric and chemiluminescent assays for the highly sensitive and specific detection of cancer protein markers, alpha-fetoprotein (AFP) and prostate specific antigen (PSA). The colorimetric CRCAS assay is 4000 times more sensitive than a conventional enzyme-linked immunosorbent assay (ELISA), and its concentration range is 10 000 times wider. Control experiments show that as low as 10 pg mL(-1) AFP or PSA could be detected even in the presence of interfering protein markers with a more than 10(5)-fold greater concentration in the sample, demonstrating the high specificity of the CRCAS assay. The limit of detection of the chemiluminescent CRCAS assays for AFP and PSA are 5 fg mL(-1) (70 aM) and 10 fg mL(-1) (0.29 fM), respectively, indicating that the sensitivity is much higher than that of the colorimetric CRCAS assay. Importantly, CRCAS works well with real biological samples.
收录类别	SCI
语种	英语
WOS记录号	WOS:000327792600017
公开日期	2014-06-13
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/13684]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Zhao, Bin,Yan, Juan,Wang, Dongfang,et al. Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers[J]. SMALL,2013,9(15):CONCATENATE(Sheet1!I47,-Sheet1!J47).
APA	Zhao, Bin.,Yan, Juan.,Wang, Dongfang.,Ge, Zhilei.,He, Shijiang.,...&Fan, Chunhai.(2013).Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers.SMALL,9(15),CONCATENATE(Sheet1!I47,-Sheet1!J47).
MLA	Zhao, Bin,et al."Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers".SMALL 9.15(2013):CONCATENATE(Sheet1!I47,-Sheet1!J47).