Analysis of the selectivity filter of the voltage-gated sodium channel NavRh

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Analysis of the selectivity filter of the voltage-gated sodium channel NavRh
	Zhang, Xu ; Xia, Mengdie ; Li, Yang ; Liu, Huihui ; Jiang, Xin ; Ren, Wenlin ; Wu, Jianping ; DeCaen, Paul ; Yu, Feng（郁锋） ; Huang, Sheng ; He, Jianhua（何建华） ; Clapham, David E. ; Yan, Nieng ; Gong, Haipeng
刊名	CELL RESEARCH
	2013
卷号	23 期号:3 页码:CONCATENATE(Sheet1!I29,-Sheet1!J29)
ISSN号	1001-0602
英文摘要	NaChBac is a bacterial voltage-gated sodium (Na-v) channel that shows sequence similarity to voltage-gated calcium channels. To understand the ion-permeation mechanism of Na-v channels, we combined molecular dynamics simulation, structural biology and electrophysiological approaches to investigate the recently determined structure of NavRh, a marine bacterial NaChBac ortholog. Two Na+ binding sites are identified in the selectivity filter (SF) in our simulations: The extracellular Na+ ion first approaches site 1 constituted by the side groups of Ser181 and Glu183, and then spontaneously arrives at the energetically more favorable site 2 formed by the carbonyl oxygens of Leu179 and Thr178. In contrast, Ca2+ ions are prone to being trapped by Glu183 at site 1, which then blocks the entrance of both Na+ and Ca2+ to the vestibule of the SF. In addition, Na+ permeates through the selective filter in an asymmetrical manner, a feature that resembles that of the mammalian Na-v orthologs. The study reported here provides insights into the mechanism of ion selectivity on Na+ over Ca2+ in mammalian Na-v channels.
收录类别	SCI
语种	英语
WOS记录号	WOS:000316375000013
公开日期	2014-06-13
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/13668]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Zhang, Xu,Xia, Mengdie,Li, Yang,et al. Analysis of the selectivity filter of the voltage-gated sodium channel NavRh[J]. CELL RESEARCH,2013,23(3):CONCATENATE(Sheet1!I29,-Sheet1!J29).
APA	Zhang, Xu.,Xia, Mengdie.,Li, Yang.,Liu, Huihui.,Jiang, Xin.,...&Gong, Haipeng.(2013).Analysis of the selectivity filter of the voltage-gated sodium channel NavRh.CELL RESEARCH,23(3),CONCATENATE(Sheet1!I29,-Sheet1!J29).
MLA	Zhang, Xu,et al."Analysis of the selectivity filter of the voltage-gated sodium channel NavRh".CELL RESEARCH 23.3(2013):CONCATENATE(Sheet1!I29,-Sheet1!J29).