Gold nanoparticle-assisted primer walking for closing the human chromosomal gap

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	Gold nanoparticle-assisted primer walking for closing the human chromosomal gap
	Li, Haikuo ; Shi, Baochen ; Li, Xueling ; Nyegaard, Steffen ; Lu, Ming ; Lu, Junhong ; Cao, Xueyan ; Huang, Qing（黄庆） ; Fan, Chunhai（樊春海） ; Chen, Runsheng ; Hu, Jun（胡钧）
刊名	ANALYTICAL METHODS
	2013
卷号	5 期号:18 页码:CONCATENATE(Sheet1!I139,-Sheet1!J139)
ISSN号	1759-9660
英文摘要	The finished sequence of the human genome still contains 260 euchromatic gaps. All the PCR-based genome walking techniques used to close gaps have common limitations, such as low efficiency and low specificity. We herein describe a strategy to solve this problem by employing gold nanoparticles (AuNPs) to improve the efficiency in primer walking amplification. We used this strategy to close a gap in human chromosome 5 containing a DNA stretch composed of the 12SAT repeat. The obtained gap sequence is highly conserved among several mammalian genomes. The demonstrated AuNP-assisted primer walking strategy is capable of effectively improving the specificity of PCR amplification and enriching the yield of target DNA fragments; it offers a new avenue for closing gaps left by current sequencing methods.
收录类别	SCI
语种	英语
WOS记录号	WOS:000323518200029
公开日期	2014-06-13
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/13618]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Li, Haikuo,Shi, Baochen,Li, Xueling,et al. Gold nanoparticle-assisted primer walking for closing the human chromosomal gap[J]. ANALYTICAL METHODS,2013,5(18):CONCATENATE(Sheet1!I139,-Sheet1!J139).
APA	Li, Haikuo.,Shi, Baochen.,Li, Xueling.,Nyegaard, Steffen.,Lu, Ming.,...&Hu, Jun.(2013).Gold nanoparticle-assisted primer walking for closing the human chromosomal gap.ANALYTICAL METHODS,5(18),CONCATENATE(Sheet1!I139,-Sheet1!J139).
MLA	Li, Haikuo,et al."Gold nanoparticle-assisted primer walking for closing the human chromosomal gap".ANALYTICAL METHODS 5.18(2013):CONCATENATE(Sheet1!I139,-Sheet1!J139).