失重飞机上进行的电泳分离实验

	失重飞机上进行的电泳分离实验
	蒋远大 ; 张志远 ; 王海波 ; 刘文喜 ; 周顺林 ; 冯学章 ; 吴汉基
刊名	空间科学学报
	2003
卷号	23 期号:3 页码:233-239
关键词	电泳分离实验微重力环境生物材料飞行试验飞机重力变化
ISSN号	0254-6124
其他题名	THE ELECTROPHORETIC SEPARATION EXPERIMENTS ON THE WEIGHTLESS AIRCRAFT
通讯作者	北京8701信箱
中文摘要	介绍了用A3—l连续自由流电泳仪在俄罗斯失重飞机上进行的实验情况及结果．进行了4个架次的飞行试验，获得了飞行试验的数据和电泳分离图像．试验结果表明，电泳仪装置工作良好，从分离图像可以看出重力变化对电泳分离过程的影响并证实微重力环境对生物材料的电泳分离是有利的．
英文摘要	This paper presents the separation experiments and results by a A3-1 contin-uous free-flow electrophoresis device on board the Russian weightless aircraft. The A3-1 device consists of power conditioning unit, detection unit and electrophore-sis unit. The power conditioning unit converts 27 VDC coming from the ?pus into the power sources required for electrophoresis unit. ThP detection un}.1 ha;v a video tape recorder used for recording the separation picture and a Jimg1chip which con-trol the power supply, the detecting, record and storage of operwc.ing aata. The electrophoresis unit is a electrophorrai:: separation in which the rectangu-lar separation chaml:1_as thf} following irarai dimensions: 270mm long, 60mm wide. a ;1.i }mn thick (gap,.LOrring operation of A3-1 device, the separation process is mc,nitre}i with.CD head placed the front of separation chamber and recorded in the video tape recorder (Video Hi8) in the detection unit. The carrier buffer (the electrode buffer is the same)，used for all experiments, contains 3.5 mMol TEA (Triethanolamine), 0.7 mMol NaAc and 10% glycerol. The sample to be separated is a kind of mixture containing two colored model proteins, Hemoglobin (HB, yellow) and Cytochrome c (red). The flight test was performed for tour times. For each flight the aircraft would conduct 10-12 parabolic flights and lasted nearly one hour. The pictures of elec-trophoretic separation and the experimental data were obtained. The results showed that the A3-1 CFFE device worked well and the effect of gravity change on the elec- trophoretic separation process of biological samples can be seen clearly from the pictures. The shape of sample filament is different under different flight state. The filament under the horizontal flight (near 1。)appears spread and bent to the right near the outlet. The filament under the overweight (1-2 g) flight is not clear due to serious spread, the separation of sample was bad. But the filament under the micro- gravity (0-0.4 g) condition is quite clear (less spread) and almost in a straight line. It was shown that the microgravity environment is beneficial to the electrophoresis separation of biological material.
学科主题	空间技术
收录类别	CSCD
资助信息	国家载人航天工程
语种	中文
CSCD记录号	CSCD:1151863
内容类型	期刊论文
源URL	[http://ir.cssar.ac.cn/handle/122/624]
专题	国家空间科学中心_空间技术部
推荐引用方式 GB/T 7714	蒋远大,张志远,王海波,等. 失重飞机上进行的电泳分离实验[J]. 空间科学学报,2003,23(3):233-239.
APA	蒋远大.,张志远.,王海波.,刘文喜.,周顺林.,...&吴汉基.(2003).失重飞机上进行的电泳分离实验.空间科学学报,23(3),233-239.
MLA	蒋远大,et al."失重飞机上进行的电泳分离实验".空间科学学报 23.3(2003):233-239.