Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics

CORC > 沈阳自动化研究所 > 中国科学院沈阳自动化研究所 > 机器人学研究室

	Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics
	Liu N(刘娜); Liang WF(梁文峰); Liu LQ(刘连庆); Wang YC(王越超); Mai, John D.; Lee, Gwo-Bin; Li WJ(李文荣)
刊名	LAB ON A CHIP
	2014
卷号	14 期号:7 页码:1367-1376
ISSN号	1473-0197
通讯作者	刘连庆 ; 李文荣
产权排序	1
中文摘要	The culturing of cancer cells on micropatterned substrates can provide insight into the factors of the extracellular environment that enable the control of cell growth. We report here a novel non-UV-based technique to quickly micropattern a poly-(ethylene) glycol diacrylate (PEGDA)-based hydrogel on top of modified glass substrates, which were then used to control the growth patterns of breast cancer cells. Previously, the fabrication of micropatterned substrates required relatively complicated steps, which made it impractical for researchers to rapidly and systematically investigate the effects of different cell growth patterns. The technique presented herein operates on the principle of optically-induced electrokinetics (OEKs) and uses computer-generated projection light patterns to dynamically pattern the hydrogel on a hydrogenated amorphous silicon (a-Si: H) thin-film, atop an indium tin oxide (ITO) glass substrate. This technique allows us to pattern lines, circles, pentagons, and more complex shapes in the hydrogel with line widths below 3 mu m and thicknesses of up to 6 mu m within 8 s by simply controlling the projected illumination pattern and applying an appropriate AC voltage between the two ITO glass substrates. After separating the glass substrates to expose the patterned hydrogel, we experimentally demonstrate that MCF-7 breast cancer cells will adhere to the bare a-Si:H surface, but not to the hydrogel patterned in various geometric shapes and sizes. Theoretical analysis and finite-element model simulations reveal that the dominant OEK forces in our technique are the dielectrophoresis (DEP) force and the electro-osmosis force, which enhance the photo-initiated cross-linking reaction in the hydrogel. Our preliminary cultures of breast cancer cells demonstrate that this reported technique could be applied to effectively confine the growth of cancer cells on a-Si:H surfaces and affect individual cell geometry during their growth.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]	MIGRATION ; DIELECTROPHORESIS ; FORCE ; MICROPARTICLES ; MANIPULATION ; STIFFNESS ; PROTEINS ; PLATFORM ; CULTURE
收录类别	SCI
语种	英语
WOS记录号	WOS:000332454600015
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/14725]
专题	沈阳自动化研究所_机器人学研究室
推荐引用方式 GB/T 7714	Liu N,Liang WF,Liu LQ,et al. Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics[J]. LAB ON A CHIP,2014,14(7):1367-1376.
APA	Liu N.,Liang WF.,Liu LQ.,Wang YC.,Mai, John D..,...&Li WJ.(2014).Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics.LAB ON A CHIP,14(7),1367-1376.
MLA	Liu N,et al."Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics".LAB ON A CHIP 14.7(2014):1367-1376.