Purification and Characteristics of Xyloglucanase and Five Other Cellulolytic Enzymes from Trichoderma reesei QM9414. 78(4), 424-430

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 生物传感技术团队

	Purification and Characteristics of Xyloglucanase and Five Other Cellulolytic Enzymes from Trichoderma reesei QM9414. 78(4), 424-430
	Qi, H ; Bai, F ; Liu, A.
刊名	Biochemistry (Mosc)
	2013
卷号	78 期号:4 页码:424-430
关键词	Candida kutaonensis sp. Nov Conserved Motif Inulinase Purification Yeast
中文摘要	A novel extracellular exoinulinase was purified and characterized from a new yeast strain KRF1T, and the gene encoding the enzyme was successfully cloned. The enzyme was stable at low pH between 3.0 and 6.5. The Km and Vmax values of the purified enzyme for inulin were 2.3 mg/mL and 4.8 mg/min, respectively. The optimum temperature of the inulinase was 50 °C, and the enzyme remained 78 % of activity at 60 °C for 2 h. The inulinase showed an amino acid sequence identity of 58 % to its closest homolog in Meyerozyma (Pichia) guilliermondii. In the secondary structure, the domain G (VMEVH) of the enzyme contained three unique residues (V, M, and H). Compared with previously reported inulinases, the enzyme from strain KRF1T displayed strong acid resistance, notable thermostability, and high affinity for the substrate of inulin. Based on sequence analysis of the 26S rDNA D1/D2 domain and phenotypic characterization, the yeast strain KRF1T was found to represent a novel anamorphic, ascomycetous yeast species. A complete description of the species is given and the name Candida kutaonensis sp. nov (type strain0KRF1T0AS 2.4027T0CBS 11388T) is proposed.
学科主题	生物传感器技术
收录类别	SCI
语种	英语
公开日期	2014-03-27
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1759]
专题	青岛生物能源与过程研究所_生物传感技术团队
推荐引用方式 GB/T 7714	Qi, H,Bai, F,Liu, A.. Purification and Characteristics of Xyloglucanase and Five Other Cellulolytic Enzymes from Trichoderma reesei QM9414. 78(4), 424-430[J]. Biochemistry (Mosc),2013,78(4):424-430.
APA	Qi, H,Bai, F,&Liu, A..(2013).Purification and Characteristics of Xyloglucanase and Five Other Cellulolytic Enzymes from Trichoderma reesei QM9414. 78(4), 424-430.Biochemistry (Mosc),78(4),424-430.
MLA	Qi, H,et al."Purification and Characteristics of Xyloglucanase and Five Other Cellulolytic Enzymes from Trichoderma reesei QM9414. 78(4), 424-430".Biochemistry (Mosc) 78.4(2013):424-430.